398d: Difference between revisions

Line 1:

[[Image:398d.gif|left|200px]]<br />

[[Image:398d.gif|left|200px]]<br /><applet load="398d" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="398d" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="398d, resolution 1.940Å" />

'''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''<br />

==Overview==

During initiation of minus-strand synthesis by HIV-1 reverse, transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of, tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand, specifically, opposite the newly synthesized DNA strand. We have, determined the crystal structure at 1.9 A resolution of an eight-base pair, hybrid duplex representing the junction to identify global or local, structural perturbations which may be recognized by HIV-RT RNase H. The, junction octamer is in a global A-type conformation throughout. A base, pair step with distinct stacking geometry and variable backbone, conformation is located next to the main endonucleolytic cleavage site., This base pair step may serve as a recognition site for HIV-RT RNase H.

During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.

==About this Structure==

398D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=398D OCA].

398D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA].

==Reference==

Line 21:

Line 20:

[[Category: double helix]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on Thu ~~Nov 8 14~~:57:~~59 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:37 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:398d.gif|left|200px]]<br />
+[[Image:398d.gif|left|200px]]<br /><applet load="398d" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="398d" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="398d, resolution 1.940&Aring;" />
 '''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''<br />
 ==Overview==
-During initiation of minus-strand synthesis by HIV-1 reverse, transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of, tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand, specifically, opposite the newly synthesized DNA strand. We have, determined the crystal structure at 1.9 A resolution of an eight-base pair, hybrid duplex representing the junction to identify global or local, structural perturbations which may be recognized by HIV-RT RNase H. The, junction octamer is in a global A-type conformation throughout. A base, pair step with distinct stacking geometry and variable backbone, conformation is located next to the main endonucleolytic cleavage site., This base pair step may serve as a recognition site for HIV-RT RNase H.
+During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.
 ==About this Structure==
-D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=398D OCA].
+D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA].
 ==Reference==
@@ Line 21: / Line 20: @@
 [[Category: double helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 14:57:59 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:37 2008''