1dsw: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:1dsw.gif|left|200px]]
{{Seed}}
[[Image:1dsw.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1dsw|  PDB=1dsw  |  SCENE=  }}  
{{STRUCTURE_1dsw|  PDB=1dsw  |  SCENE=  }}  


'''THE SOLUTION STRUCTURE OF A MONOMERIC, REDUCED FORM OF HUMAN COPPER, ZINC SUPEROXIDE DISMUTASE BEARING THE SAME CHARGE AS THE NATIVE PROTEIN'''
===THE SOLUTION STRUCTURE OF A MONOMERIC, REDUCED FORM OF HUMAN COPPER, ZINC SUPEROXIDE DISMUTASE BEARING THE SAME CHARGE AS THE NATIVE PROTEIN===




==Overview==
<!--
The solution structure of a mutated (Phe50Glu, Gly51Glu, Val148Lys, Ile151Lys), reduced, monomeric form of human copper,zinc superoxide dismutase (SOD; 153 amino acids) has been determined through 2237 meaningful nuclear Overhauser enhancements, out of 2492, and 43 dihedral angle constraints. A characteristic of this mutant is that of having the same overall charge as the dimeric protein, but an activity of only 20% with respect to wild-type SOD. This protein, at variance with a previously characterized monomeric form (Phe50Glu, Gly51Glu, Glu133Gln), does not contain mutations in the active site. Therefore, its characterization allows us to understand the structural changes independently induced by the monomerization and by the active site mutation. The family of 36 conformers, which have a target function with respect to the experimental constraints lower than 1.5 A2, has RMSD values with respect to the average structure of 0.94 +/- 0.14 A2 and 1.50 +/- 0.14 A2 for the backbone and the heavy atoms, respectively. The overall folding, which includes the classical eight-stranded Greek-key beta-barrel and a short alpha-helix, is very close to that of the previously characterized monomeric mutant E133QM2SOD and to that of wild-type SOD. The region involved in the subunit-subunit interactions in the dimeric protein is confirmed to be disordered in the monomeric species. It is also observed that a sizable rearrangement of the charged groups of the electrostatic loop and of Arg143 takes place in the monomeric species. The width of the active site channel, both at its entrance and at the bottleneck of the active site, is discussed in the light of the influence on the enzymatic activity and the latter with respect to the overall charge. It is also confirmed that the NH proton of His63 shields the Cu(I) from the bulk solvent, thus supporting the suggestion that superoxide may interact with the reduced metal ion in an outer-sphere fashion.
The line below this paragraph, {{ABSTRACT_PUBMED_10631612}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10631612 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_10631612}}


==About this Structure==
==About this Structure==
1DSW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DSW OCA].  
1DSW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DSW OCA].  


==Reference==
==Reference==
Line 32: Line 36:
[[Category: Reduced copper-zinc-protein]]
[[Category: Reduced copper-zinc-protein]]
[[Category: Single alpha helix]]
[[Category: Single alpha helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:14:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:34:05 2008''

Revision as of 20:34, 30 June 2008

File:1dsw.png

Template:STRUCTURE 1dsw

THE SOLUTION STRUCTURE OF A MONOMERIC, REDUCED FORM OF HUMAN COPPER, ZINC SUPEROXIDE DISMUTASE BEARING THE SAME CHARGE AS THE NATIVE PROTEIN

Template:ABSTRACT PUBMED 10631612

About this Structure

1DSW is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

The solution structure of a monomeric, reduced form of human copper,zinc superoxide dismutase bearing the same charge as the native protein., Banci L, Bertini I, Del Conte R, Fadin R, Mangani S, Viezzoli MS, J Biol Inorg Chem. 1999 Dec;4(6):795-803. PMID:10631612

Page seeded by OCA on Mon Jun 30 23:34:05 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1dsw&oldid=588974"