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<StructureSection load='2fnp' size='340' side='right'caption='[[2fnp]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='2fnp' size='340' side='right'caption='[[2fnp]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2fnp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FNP FirstGlance]. <br>
<table><tr><td colspan='2'>[[2fnp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FNP FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sarA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fnp OCA], [https://pdbe.org/2fnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fnp RCSB], [https://www.ebi.ac.uk/pdbsum/2fnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fnp ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fnp OCA], [https://pdbe.org/2fnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fnp RCSB], [https://www.ebi.ac.uk/pdbsum/2fnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fnp ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/SARA_STAAW SARA_STAAW]] Global regulator with both positive and negative effects that controls the expression of several virulence factors and the biofilm formation process in a cell density-dependent manner (By similarity). Required for transcription of primary transcripts RNAII and RNAIII generated by agr (virulence accessory gene regulator) locus. Negatively regulates the expression of spa (protein A) and aur (metalloprotease aureolysin).  
[https://www.uniprot.org/uniprot/SARA_STAAW SARA_STAAW] Global regulator with both positive and negative effects that controls the expression of several virulence factors and the biofilm formation process in a cell density-dependent manner (By similarity). Required for transcription of primary transcripts RNAII and RNAIII generated by agr (virulence accessory gene regulator) locus. Negatively regulates the expression of spa (protein A) and aur (metalloprotease aureolysin).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fnp ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fnp ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The sarA locus in Staphylococcus aureus controls the expression of many virulence genes. The sarA regulatory molecule, SarA, is a 14.7-kDa protein (124 residues) that binds to the promoter region of target genes. Here we report the 2.6 A-resolution x-ray crystal structure of the dimeric winged helix SarA protein, which differs from the published SarA structure dramatically. In the crystal packing, multiple dimers of SarA form a scaffold, possibly via divalent cations. Mutations of individual residues within the DNA-binding helix-turn-helix and the winged region as well as within the metal-binding pocket implicate basic residues R84 and R90 within the winged region to be critical in DNA binding, whereas acidic residues D88 and E89 (wing), D8 and E11 (metal-binding pocket), and cysteine 9 are essential for SarA function. These data suggest that the winged region of the winged helix protein participates in DNA binding and activation, whereas the putative divalent cation binding pocket is only involved in gene function.
Structural and function analyses of the global regulatory protein SarA from Staphylococcus aureus.,Liu Y, Manna AC, Pan CH, Kriksunov IA, Thiel DJ, Cheung AL, Zhang G Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2392-7. Epub 2006 Feb 2. PMID:16455801<ref>PMID:16455801</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2fnp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cheung, A L]]
[[Category: Staphylococcus aureus]]
[[Category: Liu, Y]]
[[Category: Cheung AL]]
[[Category: Manna, A C]]
[[Category: Liu Y]]
[[Category: Pan, C H]]
[[Category: Manna AC]]
[[Category: Zhang, G]]
[[Category: Pan CH]]
[[Category: Dna binding]]
[[Category: Zhang G]]
[[Category: Transcription]]
[[Category: Wing-helix]]
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