1dxd: Difference between revisions

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[[Image:1dxd.gif|left|200px]]
{{Seed}}
[[Image:1dxd.png|left|200px]]


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{{STRUCTURE_1dxd|  PDB=1dxd  |  SCENE=  }}  
{{STRUCTURE_1dxd|  PDB=1dxd  |  SCENE=  }}  


'''PHOTOLYZED CO COMPLEX OF MYOGLOBIN MB-YQR AT 20K'''
===PHOTOLYZED CO COMPLEX OF MYOGLOBIN MB-YQR AT 20K===




==Overview==
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We determined the structure of the photolytic intermediate of a sperm whale myoglobin (Mb) mutant called Mb-YQR [Leu-(B10)--&gt;Tyr; His(E7)--&gt;Gln; Thr(E10)--&gt;Arg] to 1.4-A resolution by ultra-low temperature (20 K) x-ray diffraction. Starting with the CO complex, illumination leads to photolysis of the Fe-CO bond, and migration of the photolyzed carbon monoxide (CO*) to a niche in the protein 8.1 A from the heme iron; this cavity corresponds to that hosting an atom of Xe when the crystal is equilibrated with xenon gas at 7 atmospheres [Tilton, R. F., Jr., Kuntz, I. D. &amp; Petsko, G. A. (1984) Biochemistry 23, 2849-2857]. The site occupied by CO* corresponds to that predicted by molecular dynamics simulations previously carried out to account for the NO geminate rebinding of Mb-YQR observed in laser photolysis experiments at room temperature. This secondary docking site differs from the primary docking site identified by previous crystallographic studies on the photolyzed intermediate of wild-type sperm whale Mb performed at cryogenic temperatures [Teng et al. (1994) Nat. Struct. Biol. 1, 701-705] and room temperature [Srajer et al. (1996) Science 274, 1726-1729]. Our experiment shows that the pathway of a small molecule in its trajectory through a protein may be modified by site-directed mutagenesis, and that migration within the protein matrix to the active site involves a limited number of pre-existing cavities identified in the interior space of the protein.
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{{ABSTRACT_PUBMED_10681426}}


==About this Structure==
==About this Structure==
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[[Category: Oxygen storage]]
[[Category: Oxygen storage]]
[[Category: Respiratory protein]]
[[Category: Respiratory protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:23:39 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:45:35 2008''

Revision as of 20:45, 30 June 2008

File:1dxd.png

Template:STRUCTURE 1dxd

PHOTOLYZED CO COMPLEX OF MYOGLOBIN MB-YQR AT 20K

Template:ABSTRACT PUBMED 10681426

About this Structure

1DXD is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

Reference

The role of cavities in protein dynamics: crystal structure of a photolytic intermediate of a mutant myoglobin., Brunori M, Vallone B, Cutruzzola F, Travaglini-Allocatelli C, Berendzen J, Chu K, Sweet RM, Schlichting I, Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2058-63. PMID:10681426

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