7bfp: Difference between revisions
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==== | ==Structure of the Integrator cleavage module with INTS4/9/11== | ||
<StructureSection load='7bfp' size='340' side='right'caption='[[7bfp]]' scene=''> | <StructureSection load='7bfp' size='340' side='right'caption='[[7bfp]], [[Resolution|resolution]] 3.56Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7bfp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BFP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bfp OCA], [https://pdbe.org/7bfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bfp RCSB], [https://www.ebi.ac.uk/pdbsum/7bfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bfp ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.56Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bfp OCA], [https://pdbe.org/7bfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bfp RCSB], [https://www.ebi.ac.uk/pdbsum/7bfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bfp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/INT9_HUMAN INT9_HUMAN] Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Integrator is a specialized 3' end-processing complex involved in cleavage and transcription termination of a subset of nascent RNA polymerase II transcripts, including small nuclear RNAs (snRNAs). We provide evidence of the modular nature of the Integrator complex by biochemically characterizing its two subcomplexes, INTS5/8 and INTS10/13/14. Using cryoelectron microscopy (cryo-EM), we determined a 3.5-A-resolution structure of the INTS4/9/11 ternary complex, which constitutes Integrator's catalytic core. Our structure reveals the spatial organization of the catalytic nuclease INTS11, bound to its catalytically impaired homolog INTS9 via several interdependent interfaces. INTS4, a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. In this assembly, all three proteins form a composite electropositive groove, suggesting a putative RNA binding path within the complex. Comparison with other 3' end-processing machineries points to distinct features and a unique architecture of the Integrator's catalytic module. | |||
Structure of the catalytic core of the Integrator complex.,Pfleiderer MM, Galej WP Mol Cell. 2021 Mar 18;81(6):1246-1259.e8. doi: 10.1016/j.molcel.2021.01.005. Epub , 2021 Feb 5. PMID:33548203<ref>PMID:33548203</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7bfp" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Integrator complex 3D structures|Integrator complex 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Galej WP]] | ||
[[Category: Pfleiderer MM]] | |||
