1a17: Difference between revisions

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[[Image:1a17.gif|left|200px]]<br />

[[Image:1a17.gif|left|200px]]<br /><applet load="1a17" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1a17" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1a17, resolution 2.45Å" />

'''TETRATRICOPEPTIDE REPEATS OF PROTEIN PHOSPHATASE 5'''<br />

==Overview==

The tetratricopeptide repeat (TPR) is a degenerate 34 amino acid sequence, identified in a wide variety of proteins, present in tandem arrays of 3-16, motifs, which form scaffolds to mediate protein-protein interactions and, often the assembly of multiprotein complexes. TPR-containing proteins, include the anaphase promoting complex (APC) subunits cdc16, cdc23 and, cdc27, the NADPH oxidase subunit p67 phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, and peroxisomal, and mitochondrial import proteins. Here, we report the crystal structure, of the TPR domain of a protein phosphatase, PP5. Each of the three TPR, motifs of this domain consist of a pair of antiparallel alpha-helices of, equivalent length. Adjacent TPR motifs are packed together in a parallel, arrangement such that a tandem TPR motif structure is composed of a, regular series of antiparallel alpha-helices. The uniform angular and, spatial arrangement of neighbouring alpha-helices defines a helical, structure and creates an amphipathic groove. Multiple-TPR motif proteins, would fold into a right-handed super-helical structure with a continuous, helical groove suitable for the recognition of target proteins, hence, defining a novel mechanism for protein recognition. The spatial, arrangement of alpha-helices in the PP5-TPR domain is similar to those, within 14-3-3 proteins.

The tetratricopeptide repeat (TPR) is a degenerate 34 amino acid sequence identified in a wide variety of proteins, present in tandem arrays of 3-16 motifs, which form scaffolds to mediate protein-protein interactions and often the assembly of multiprotein complexes. TPR-containing proteins include the anaphase promoting complex (APC) subunits cdc16, cdc23 and cdc27, the NADPH oxidase subunit p67 phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, and peroxisomal and mitochondrial import proteins. Here, we report the crystal structure of the TPR domain of a protein phosphatase, PP5. Each of the three TPR motifs of this domain consist of a pair of antiparallel alpha-helices of equivalent length. Adjacent TPR motifs are packed together in a parallel arrangement such that a tandem TPR motif structure is composed of a regular series of antiparallel alpha-helices. The uniform angular and spatial arrangement of neighbouring alpha-helices defines a helical structure and creates an amphipathic groove. Multiple-TPR motif proteins would fold into a right-handed super-helical structure with a continuous helical groove suitable for the recognition of target proteins, hence defining a novel mechanism for protein recognition. The spatial arrangement of alpha-helices in the PP5-TPR domain is similar to those within 14-3-3 proteins.

==About this Structure==

1A17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1A17 OCA].

1A17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A17 OCA].

==Reference==

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[[Category: Single protein]]

[[Category: Barford, D.]]

[[Category: Cohen, P.T.W.]]

[[Category: Cohen, P T.W.]]

[[Category: Das, A.K.]]

[[Category: Das, A K.]]

[[Category: SO4]]

[[Category: hydrolase]]

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[[Category: x-ray structure]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 15~~:54:~~02 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:42 2008''

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-[[Image:1a17.gif|left|200px]]<br />
+[[Image:1a17.gif|left|200px]]<br /><applet load="1a17" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1a17" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1a17, resolution 2.45&Aring;" />
 '''TETRATRICOPEPTIDE REPEATS OF PROTEIN PHOSPHATASE 5'''<br />
 ==Overview==
-The tetratricopeptide repeat (TPR) is a degenerate 34 amino acid sequence, identified in a wide variety of proteins, present in tandem arrays of 3-16, motifs, which form scaffolds to mediate protein-protein interactions and, often the assembly of multiprotein complexes. TPR-containing proteins, include the anaphase promoting complex (APC) subunits cdc16, cdc23 and, cdc27, the NADPH oxidase subunit p67 phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, and peroxisomal, and mitochondrial import proteins. Here, we report the crystal structure, of the TPR domain of a protein phosphatase, PP5. Each of the three TPR, motifs of this domain consist of a pair of antiparallel alpha-helices of, equivalent length. Adjacent TPR motifs are packed together in a parallel, arrangement such that a tandem TPR motif structure is composed of a, regular series of antiparallel alpha-helices. The uniform angular and, spatial arrangement of neighbouring alpha-helices defines a helical, structure and creates an amphipathic groove. Multiple-TPR motif proteins, would fold into a right-handed super-helical structure with a continuous, helical groove suitable for the recognition of target proteins, hence, defining a novel mechanism for protein recognition. The spatial, arrangement of alpha-helices in the PP5-TPR domain is similar to those, within 14-3-3 proteins.
+The tetratricopeptide repeat (TPR) is a degenerate 34 amino acid sequence identified in a wide variety of proteins, present in tandem arrays of 3-16 motifs, which form scaffolds to mediate protein-protein interactions and often the assembly of multiprotein complexes. TPR-containing proteins include the anaphase promoting complex (APC) subunits cdc16, cdc23 and cdc27, the NADPH oxidase subunit p67 phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, and peroxisomal and mitochondrial import proteins. Here, we report the crystal structure of the TPR domain of a protein phosphatase, PP5. Each of the three TPR motifs of this domain consist of a pair of antiparallel alpha-helices of equivalent length. Adjacent TPR motifs are packed together in a parallel arrangement such that a tandem TPR motif structure is composed of a regular series of antiparallel alpha-helices. The uniform angular and spatial arrangement of neighbouring alpha-helices defines a helical structure and creates an amphipathic groove. Multiple-TPR motif proteins would fold into a right-handed super-helical structure with a continuous helical groove suitable for the recognition of target proteins, hence defining a novel mechanism for protein recognition. The spatial arrangement of alpha-helices in the PP5-TPR domain is similar to those within 14-3-3 proteins.
 ==About this Structure==
-A17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A17 OCA].
+A17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A17 OCA].
 ==Reference==
@@ Line 16: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Barford, D.]]
-[[Category: Cohen, P.T.W.]]
+[[Category: Cohen, P T.W.]]
-[[Category: Das, A.K.]]
+[[Category: Das, A K.]]
 [[Category: SO4]]
 [[Category: hydrolase]]
@@ Line 26: / Line 25: @@
 [[Category: x-ray structure]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:54:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:42 2008''