2i48: Difference between revisions

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<StructureSection load='2i48' size='340' side='right'caption='[[2i48]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='2i48' size='340' side='right'caption='[[2i48]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2i48]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aphanocapsa_sp._(strain_n-1) Aphanocapsa sp. (strain n-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I48 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2i48]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I48 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2i49|2i49]], [[2i4b|2i4b]], [[2i4c|2i4c]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cmpA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1148 Aphanocapsa sp. (strain N-1)])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i48 OCA], [https://pdbe.org/2i48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i48 RCSB], [https://www.ebi.ac.uk/pdbsum/2i48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i48 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i48 OCA], [https://pdbe.org/2i48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i48 RCSB], [https://www.ebi.ac.uk/pdbsum/2i48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i48 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/CMPA_SYNY3 CMPA_SYNY3]] Part of the ABC transporter complex CmpABCD involved in bicarbonate transport. Binds bicarbonate with high affinity (By similarity).  
[https://www.uniprot.org/uniprot/CMPA_SYNY3 CMPA_SYNY3] Part of the ABC transporter complex CmpABCD involved in bicarbonate transport. Binds bicarbonate with high affinity (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i48 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i48 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cyanobacteria, blue-green algae, are the most abundant autotrophs in aquatic environments and form the base of the food chain by fixing carbon and nitrogen into cellular biomass. To compensate for the low selectivity of Rubisco for CO2 over O2, cyanobacteria have developed highly efficient CO2-concentrating machinery of which the ABC transport system CmpABCD from Synechocystis PCC 6803 is one component. Here, we have described the structure of the bicarbonate-binding protein CmpA in the absence and presence of bicarbonate and carbonic acid. CmpA is highly homologous to the nitrate transport protein NrtA. CmpA binds carbonic acid at the entrance to the ligand-binding pocket, whereas bicarbonate binds in nearly an identical location compared with nitrate binding to NrtA. Unexpectedly, bicarbonate binding is accompanied by a metal ion, identified as Ca2+ via inductively coupled plasma optical emission spectrometry. The binding of bicarbonate and metal appears to be highly cooperative and suggests that CmpA may co-transport bicarbonate and calcium or that calcium acts a cofactor in bicarbonate transport.
The structure of a cyanobacterial bicarbonate transport protein, CmpA.,Koropatkin NM, Koppenaal DW, Pakrasi HB, Smith TJ J Biol Chem. 2007 Jan 26;282(4):2606-14. Epub 2006 Nov 22. PMID:17121816<ref>PMID:17121816</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2i48" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Koropatkin, N M]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Pakrasi, H B]]
[[Category: Koropatkin NM]]
[[Category: Smith, T J]]
[[Category: Pakrasi HB]]
[[Category: Abc transporter]]
[[Category: Smith TJ]]
[[Category: Alpha-beta protein]]
[[Category: Bicarbonate-binding protein]]
[[Category: C-clamp]]
[[Category: Periplasmic solute-binding protein]]
[[Category: Transport protein]]

Latest revision as of 09:01, 21 February 2024

Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with carbonic acid

2i48, resolution 1.60Å

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