6zxb: Difference between revisions
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==Diguanylate cyclase DgcR (I-site mutant) in native state== | ==Diguanylate cyclase DgcR (I-site mutant) in native state== | ||
<StructureSection load='6zxb' size='340' side='right'caption='[[6zxb]]' scene=''> | <StructureSection load='6zxb' size='340' side='right'caption='[[6zxb]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZXB FirstGlance]. <br> | <table><tr><td colspan='2'>[[6zxb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lepbp Lepbp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZXB FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zxb OCA], [https://pdbe.org/6zxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zxb RCSB], [https://www.ebi.ac.uk/pdbsum/6zxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zxb ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GH3:3-DEOXY-GUANOSINE-5-TRIPHOSPHATE'>GH3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LEPBI_p0053 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=456481 LEPBP])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zxb OCA], [https://pdbe.org/6zxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zxb RCSB], [https://www.ebi.ac.uk/pdbsum/6zxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zxb ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Diguanylate cyclases synthesising the bacterial second messenger c-di-GMP are found to be regulated by a variety of sensory input domains that control the activity of their catalytical GGDEF domain, but how activation proceeds mechanistically is, apart from a few examples, still largely unknown. As part of two-component systems, they are activated by cognate histidine kinases that phosphorylate their Rec input domains. DgcR from Leptospira biflexa is a constitutively dimeric prototype of this class of diguanylate cyclases. Full-length crystal structures reveal that BeF3(-) pseudo-phosphorylation induces a relative rotation of two rigid halves in the Rec domain. This is coupled to a reorganisation of the dimeric structure with concomitant switching of the coiled-coil linker to an alternative heptad register. Finally, the activated register allows the two substrate-loaded GGDEF domains, which are linked to the end of the coiled-coil via a localised hinge, to move into a catalytically competent dimeric arrangement. Bioinformatic analyses suggest that the binary register switch mechanism is utilised by many diguanylate cyclases with N-terminal coiled-coil linkers. | |||
Activation mechanism of a small prototypic Rec-GGDEF diguanylate cyclase.,Teixeira RD, Holzschuh F, Schirmer T Nat Commun. 2021 Apr 12;12(1):2162. doi: 10.1038/s41467-021-22492-7. PMID:33846343<ref>PMID:33846343</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6zxb" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Schirmer T]] | [[Category: Lepbp]] | ||
[[Category: Teixeira | [[Category: Schirmer, T]] | ||
[[Category: Teixeira, R D]] | |||
[[Category: C-di-gmp]] | |||
[[Category: Ggdef domain]] | |||
[[Category: Leptospira]] | |||
[[Category: Rec]] | |||
[[Category: Receiver domain]] | |||
[[Category: Signaling protein]] | |||
