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==Solution NMR structure of lipoprotein spr from Escherichia coli K12. Northeast Structural Genomics target ER541-37-162==
==Solution NMR structure of lipoprotein spr from Escherichia coli K12. Northeast Structural Genomics target ER541-37-162==
<StructureSection load='2k1g' size='340' side='right'caption='[[2k1g]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2k1g' size='340' side='right'caption='[[2k1g]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2k1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jyx 2jyx]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K1G FirstGlance]. <br>
<table><tr><td colspan='2'>[[2k1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2jyx 2jyx]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K1G FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spr, yeiV, b2175, JW2163 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k1g OCA], [https://pdbe.org/2k1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k1g RCSB], [https://www.ebi.ac.uk/pdbsum/2k1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k1g ProSAT], [https://www.topsan.org/Proteins/NESGC/2k1g TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k1g OCA], [https://pdbe.org/2k1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k1g RCSB], [https://www.ebi.ac.uk/pdbsum/2k1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k1g ProSAT], [https://www.topsan.org/Proteins/NESGC/2k1g TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MEPS_ECOLI MEPS_ECOLI] A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Also has weak LD-carboxypeptidase activity on L-mDAP-D-Ala peptide bonds. Partially suppresses a prc disruption mutant.<ref>PMID:23062283</ref> <ref>PMID:9158724</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k1g ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k1g ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Escherichia coli Spr is a membrane-anchored cell wall hydrolase. The solution NMR structure of the C-terminal NlpC/P60 domain of E. coli Spr described here reveals that the protein adopts a papain-like alpha+beta fold and identifies a substrate-binding cleft featuring several highly conserved residues. The active site features a novel Cys-His-His catalytic triad that appears to be a unique structural signature of this cysteine peptidase family. Moreover, the relative orientation of these catalytic residues is similar to that observed in the analogous Ser-His-His triad, a variant of the classic Ser-His-Asp charge relay system, suggesting the convergent evolution of a catalytic mechanism in quite distinct peptidase families.
Solution NMR structure of the NlpC/P60 domain of lipoprotein Spr from Escherichia coli: structural evidence for a novel cysteine peptidase catalytic triad.,Aramini JM, Rossi P, Huang YJ, Zhao L, Jiang M, Maglaqui M, Xiao R, Locke J, Nair R, Rost B, Acton TB, Inouye M, Montelione GT Biochemistry. 2008 Sep 16;47(37):9715-7. Epub 2008 Aug 21. PMID:18715016<ref>PMID:18715016</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2k1g" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Acton, T B]]
[[Category: Acton TB]]
[[Category: Aramini, J M]]
[[Category: Aramini JM]]
[[Category: Baran, M C]]
[[Category: Baran MC]]
[[Category: Huang, Y J]]
[[Category: Huang YJ]]
[[Category: Jiang, M]]
[[Category: Jiang M]]
[[Category: Liu, J]]
[[Category: Liu J]]
[[Category: Maglaqui, M]]
[[Category: Maglaqui M]]
[[Category: Montelione, G T]]
[[Category: Montelione GT]]
[[Category: Structural genomic]]
[[Category: Rossi P]]
[[Category: Rossi, P]]
[[Category: Rost B]]
[[Category: Rost, B]]
[[Category: Swapna GVT]]
[[Category: Swapna, G V.T]]
[[Category: Xiao R]]
[[Category: Xiao, R]]
[[Category: Zhao L]]
[[Category: Zhao, L]]
[[Category: Bacterial lipoprotein]]
[[Category: Construct optimized]]
[[Category: Cysteine peptidase]]
[[Category: Lipoprotein]]
[[Category: Membrane]]
[[Category: Nesg]]
[[Category: Nplc/p60 family]]
[[Category: Palmitate]]
[[Category: PSI, Protein structure initiative]]
[[Category: Solution nmr structure]]

Latest revision as of 06:43, 1 May 2024

Solution NMR structure of lipoprotein spr from Escherichia coli K12. Northeast Structural Genomics target ER541-37-162

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