1e4p: Difference between revisions

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[[Image:1e4p.gif|left|200px]]
{{Seed}}
[[Image:1e4p.png|left|200px]]


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{{STRUCTURE_1e4p|  PDB=1e4p  |  SCENE=  }}  
{{STRUCTURE_1e4p|  PDB=1e4p  |  SCENE=  }}  


'''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE'''
===STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE===




==Overview==
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The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.
The line below this paragraph, {{ABSTRACT_PUBMED_11142381}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 11142381 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11142381}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].  


==Reference==
==Reference==
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[[Category: Rna ribozyme]]
[[Category: Rna ribozyme]]
[[Category: Substrate hairpin]]
[[Category: Substrate hairpin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:39:32 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:08:43 2008''

Revision as of 21:08, 30 June 2008

File:1e4p.png

Template:STRUCTURE 1e4p

STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE

Template:ABSTRACT PUBMED 11142381

About this Structure

Full experimental information is available from OCA.

Reference

Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:11142381

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