6uvu

Crystal structure of the AntR antimony-specific transcriptional repressor

spinqualitylabelsall models 6uvu, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6uvu is a 2 chain structure with sequence from Comamonas testosteroni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A096F4H2_COMTE Publication Abstract from PubMed The ant operon of the antimony-mining bacterium Comamonas testosterone JL40 confers resistance to Sb(III). The operon is transcriptionally regulated by the product of the first gene in the operon, antR. AntR is a member of ArsR/SmtB family of metal/metalloid-responsive repressors resistance. We purified and characterized C. testosterone AntR and demonstrated that it responds to metalloids in the order Sb(III)=methylarsenite (MAs(III)>>As(III)). The protein was crystallized, and the structure solved at 2.1 A resolution. The homodimeric structure of AntR adopts a classical ArsR/SmtB topology architecture. The protein has five cysteine residues, of which Cys103a from one monomer and Cys113b from the other monomer, are proposed to form one Sb(III) binding site, and Cys113a and Cys103b forming a second binding site. This is the first report of the structure and binding properties of a transcriptional repressor with high selectivity for environmental antimony. Functional and structural characterization of AntR, an Sb(III) responsive transcriptional repressor.,Viswanathan T, Chen J, Wu M, An L, Kandavelu P, Sankaran B, Radhakrishnan M, Li M, Rosen BP Mol Microbiol. 2021 Mar 31. doi: 10.1111/mmi.14721. PMID:33786926 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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