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| {{STRUCTURE_1e6b| PDB=1e6b | SCENE= }} | | {{STRUCTURE_1e6b| PDB=1e6b | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF A ZETA CLASS GLUTATHIONE S-TRANSFERASE FROM ARABIDOPSIS THALIANA'''
| | ===CRYSTAL STRUCTURE OF A ZETA CLASS GLUTATHIONE S-TRANSFERASE FROM ARABIDOPSIS THALIANA=== |
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| ==Overview==
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| The cis-trans isomerisation of maleylacetoacetate to fumarylacetoacetate is the penultimate step in the tyrosine/phenylalanine catabolic pathway and has recently been shown to be catalysed by glutathione S-transferase enzymes belonging to the zeta class. Given this primary metabolic role it is unsurprising that zeta class glutathione S-transferases are well conserved over a considerable period of evolution, being found in vertebrates, plants, insects and fungi. The structure of this glutathione S-transferase, cloned from Arabidopsis thaliana, has been solved by single isomorphous replacement with anomalous scattering and refined to a final crystallographic R-factor of 19.6% using data from 25.0 A to 1.65 A. The zeta class enzyme adopts the canonical glutathione S-transferase fold and forms a homodimer with each subunit consisting of 221 residues. In agreement with structures of glutathione S-transferases from the theta and phi classes, a serine residue (Ser17) is present in the active site, at a position that would allow it to stabilise the thiolate anion of glutathione. Site-directed mutagenesis of this residue confirms its importance in catalysis. In addition, the role of a highly conserved cysteine residue (Cys19) present in the active site of the zeta class glutathione S-transferase enzymes is discussed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11352584}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11352584 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11352584}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Thom, R.]] | | [[Category: Thom, R.]] |
| [[Category: Transferase]] | | [[Category: Transferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:43:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:13:16 2008'' |
