Sandbox Reserved 1674: Difference between revisions
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{{Sandbox_Reserved_BHall_Sp21}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | {{Sandbox_Reserved_BHall_Sp21}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | ||
==CTX-M Beta-Lactamase== | ==CTX-M Beta-Lactamase== | ||
CTX-M Beta Lactamase is a class A enzyme that creates drug resistance to ampicillin and cefotaxime through | CTX-M Beta Lactamase is a class A enzyme that creates drug resistance to ampicillin and cefotaxime through a two step process of deacylation and acylation. | ||
== Function of your protein == | == Function of your protein == | ||
<StructureSection load='7K2X' size='340' side='right' caption='Overall structure of CTX-M Beta-Lactamase without any important ligands. (PDB: 7K2X)' scene=''> | <StructureSection load='7K2X' size='340' side='right' caption='Overall structure of CTX-M Beta-Lactamase without any important ligands. (PDB: 7K2X)' scene=''> | ||
CTX-M Beta-Lactamase is an enzyme made to inhibit Beta-Lactam. It is found within bacteria, specifically the ''E. coli'' bacteria. Beta- Lactam binds with<scene name='87/873236/Ampicillin/1'> ampicillin</scene> and <scene name='87/873236/Cefotaxime/2'>cefotaxime</scene>, both of which are types of drugs made to fight bacterial infections. Beta-Lactam specifically attacks the lactam ring within both of these structures using a deacylation. | CTX-M Beta-Lactamase is an enzyme made to inhibit Beta-Lactam. It is found within bacteria, specifically the ''E. coli'' bacteria. Beta- Lactam binds with<scene name='87/873236/Ampicillin/1'> ampicillin</scene> and <scene name='87/873236/Cefotaxime/2'>cefotaxime</scene>, both of which are types of drugs made to fight bacterial infections. Beta-Lactam specifically attacks the lactam ring within both of these structures using a deacylation. | ||
== Biological relevance and broader implications == | == Biological relevance and broader implications == | ||
This enzyme inhibits the drug's function by breaking apart the lactam ring. This is does cause drug resistance within the E. coli bacteria making it much harder to treat. | This enzyme inhibits the drug's function by breaking apart the lactam ring. This is does cause drug resistance within the ''E. coli'' bacteria making it much harder to treat via drug therapies. | ||
== Important amino acids== | == Important amino acids== | ||
The important amino acids within the CTX-M Beta Lactamase are Ser70, Ser130, Lys234, Arg234, and Lys73. This creates the <scene name='87/873236/Active_site/2'>catalytic triad</scene> with one proton shuttle. The residue at 234 does undergo a mutation in some cases and therefore can be either a Lys, or an Arg. The catalytic triad are the residues at 70, 130, and 234. The S130 helps to cleave the amide bond and distributes a proton to the nitrogen. S70 attacks the carbonyl carbon on the lactam ring before also protonating to create an alcohol and it breaks off again. K73 does work as a proton shuttle for both parts of the reaction. | The important amino acids within the CTX-M Beta Lactamase are Ser70, Ser130, Lys234, Arg234, and Lys73. This creates the <scene name='87/873236/Active_site/2'>catalytic triad</scene> with one proton shuttle. The residue at 234 does undergo a mutation in some cases and therefore can be either a Lys, or an Arg. The catalytic triad are the residues at 70, 130, and 234. The S130 helps to cleave the amide bond and distributes a proton to the nitrogen. S70 attacks the carbonyl carbon on the lactam ring before also protonating to create an alcohol and it breaks off again. K73 does work as a proton shuttle for both parts of the reaction. | ||
