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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7ask]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>
<table><tr><td colspan='2'>[[7ask]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7ase|7ase]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q4E5Z1_TRYCC Q4E5Z1_TRYCC]
Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNAi(Met) and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9(S), 7(S), and 6(S), and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. cruzi and mass spectrometry data.


Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.,Bochler A, Querido JB, Prilepskaja T, Soufari H, Simonetti A, Del Cistia ML, Kuhn L, Ribeiro AR, Valasek LS, Hashem Y Cell Rep. 2020 Dec 22;33(12):108534. doi: 10.1016/j.celrep.2020.108534. PMID:33357443<ref>PMID:33357443</ref>
==See Also==
 
*[[Helicase 3D structures|Helicase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ask" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Trypanosoma cruzi]]
[[Category: Trypanosoma cruzi]]
[[Category: Bochler, A]]
[[Category: Bochler A]]
[[Category: Cistia, M L.Del]]
[[Category: Brito Querido J]]
[[Category: Hashem, Y]]
[[Category: Del Cistia ML]]
[[Category: Kuhn, L]]
[[Category: Hashem Y]]
[[Category: Prilepskaja, T]]
[[Category: Kuhn L]]
[[Category: Querido, J Brito]]
[[Category: Prilepskaja T]]
[[Category: Ribeiro, A Rimoldi]]
[[Category: Rimoldi Ribeiro A]]
[[Category: Soufari, H]]
[[Category: Soufari H]]
[[Category: Valasek, L S]]
[[Category: Valasek LS]]
[[Category: Factor]]
[[Category: Helicase]]
[[Category: Kinetoplastid]]
[[Category: Preinitiation]]
[[Category: Translation]]

Latest revision as of 07:45, 1 May 2024

43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase bound with ATP

7ask, resolution 4.30Å

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