1eb4: Difference between revisions

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[[Image:1eb4.jpg|left|200px]]
{{Seed}}
[[Image:1eb4.png|left|200px]]


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{{STRUCTURE_1eb4|  PDB=1eb4  |  SCENE=  }}  
{{STRUCTURE_1eb4|  PDB=1eb4  |  SCENE=  }}  


'''HISTIDINE AMMONIA-LYASE (HAL) MUTANT F329A FROM PSEUDOMONAS PUTIDA'''
===HISTIDINE AMMONIA-LYASE (HAL) MUTANT F329A FROM PSEUDOMONAS PUTIDA===




==Overview==
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Histidine ammonia-lyase requires a 4-methylidene-imidazole-5-one group (MIO) that is produced autocatalytically by a cyclization and dehydration step in a 3-residue loop of the polypeptide. The crystal structures of three mutants have been established. Two mutants were inactive and failed to form MIO, but remained unchanged elsewhere. The third mutant showed very low activity and formed MIO, although it differed from an MIO-less mutant only by an additional 329-C(beta) atom. This atom forms one constraint during MIO formation, the other being the strongly connected Asp145. An exploration of the conformational space of the MIO-forming loop showed that the cyclization is probably enforced by a mechanic compression in a late stage of chain folding and is catalyzed by a well-connected internal water molecule. The cyclization of the respective 3-residue loop of green fluorescent protein is likely to occur in a similar reaction.
The line below this paragraph, {{ABSTRACT_PUBMED_11796111}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_11796111}}


==About this Structure==
==About this Structure==
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[[Category: Histidine degradation]]
[[Category: Histidine degradation]]
[[Category: Lyase]]
[[Category: Lyase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:53:34 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:26:21 2008''

Revision as of 21:26, 30 June 2008

File:1eb4.png

Template:STRUCTURE 1eb4

HISTIDINE AMMONIA-LYASE (HAL) MUTANT F329A FROM PSEUDOMONAS PUTIDA

Template:ABSTRACT PUBMED 11796111

About this Structure

1EB4 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase., Baedeker M, Schulz GE, Structure. 2002 Jan;10(1):61-7. PMID:11796111

Page seeded by OCA on Tue Jul 1 00:26:21 2008

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