7c8m: Difference between revisions
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==Crystal structure of IscU wild-type== | ==Crystal structure of IscU wild-type== | ||
<StructureSection load='7c8m' size='340' side='right'caption='[[7c8m]]' scene=''> | <StructureSection load='7c8m' size='340' side='right'caption='[[7c8m]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8M FirstGlance]. <br> | <table><tr><td colspan='2'>[[7c8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"methanothrix_thermoacetophila"_nozhevnikova_and_chudina_1984 "methanothrix thermoacetophila" nozhevnikova and chudina 1984]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8M FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8m OCA], [https://pdbe.org/7c8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8m RCSB], [https://www.ebi.ac.uk/pdbsum/7c8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8m ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8m OCA], [https://pdbe.org/7c8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8m RCSB], [https://www.ebi.ac.uk/pdbsum/7c8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8m ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
IscU serves as a scaffold for the de novo assembly of a [2Fe-2S] cluster prior to its delivery to recipient protein. It has also been proposed that on one dimer of bacterial IscU, two [2Fe-2S] clusters can be converted into a single [4Fe-4S] cluster. However, lack of structural information about the dimeric state of IscU has hindered our understanding of the underlying mechanisms. In this study, we determine the X-ray crystal structure of IscU from the thermophilic archaeon Methanothrix thermoacetophila and demonstrate a dimer structure of IscU in which two [2Fe-2S] clusters are facing each other in close proximity at the dimer interface. Our structure also reveals for the first time that Asp40 serves as a fourth ligand to the [2Fe-2S] cluster with three Cys ligands in each monomer, consistent with previous spectroscopic data. We confirm by EPR spectroscopic analysis that in solution two adjacent [2Fe-2S] clusters in the wild-type dimer are converted to a [4Fe-4S] cluster via reductive coupling. Furthermore, we find that the H106A substitution abolishes the reductive conversion to the [4Fe-4S] cluster without structural alteration, suggesting that His106 is functionally involved in this process. Overall, these findings provide a structural explanation for the assembly and conversion of Fe-S clusters on IscU and highlight a dynamic process that advances via association and dissociation of the IscU dimer. | |||
The Structure of the Dimeric State of IscU Harboring Two Adjacent [2Fe-2S] Clusters Provides Mechanistic Insights into Cluster Conversion to [4Fe-4S].,Kunichika K, Nakamura R, Fujishiro T, Takahashi Y Biochemistry. 2021 May 25;60(20):1569-1572. doi: 10.1021/acs.biochem.1c00112., Epub 2021 May 3. PMID:33938220<ref>PMID:33938220</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7c8m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Methanothrix thermoacetophila nozhevnikova and chudina 1984]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fujishiro T]] | [[Category: Fujishiro, T]] | ||
[[Category: Kunichika K]] | [[Category: Kunichika, K]] | ||
[[Category: Takahashi Y]] | [[Category: Takahashi, Y]] | ||
[[Category: Biosynthetic protein]] | |||
[[Category: Iron-sulfur cluster biogenesis]] | |||
Revision as of 12:31, 9 June 2021
Crystal structure of IscU wild-type
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