2nd9: Difference between revisions

Revision as of 10:27, 15 March 2023

Solution structure of MapZ extracellular domain first subdomain

Structural highlights

2nd9 is a 1 chain structure with sequence from Streptococcus pneumoniae R6. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MAPZ_STRR6 Early cell division protein that marks the future cell division site and supports proper FtsZ ring positioning.[HAMAP-Rule:MF_01941]^[1] ^[2]

Publication Abstract from PubMed

Accurate placement of the bacterial division site is a prerequisite for the generation of two viable and identical daughter cells. In Streptococcus pneumoniae, the positive regulatory mechanism involving the membrane protein MapZ positions precisely the conserved cell division protein FtsZ at the cell centre. Here we characterize the structure of the extracellular domain of MapZ and show that it displays a bi-modular structure composed of two subdomains separated by a flexible serine-rich linker. We further demonstrate in vivo that the N-terminal subdomain serves as a pedestal for the C-terminal subdomain, which determines the ability of MapZ to mark the division site. The C-terminal subdomain displays a patch of conserved amino acids and we show that this patch defines a structural motif crucial for MapZ function. Altogether, this structure-function analysis of MapZ provides the first molecular characterization of a positive regulatory process of bacterial cell division.

Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ.,Manuse S, Jean NL, Guinot M, Lavergne JP, Laguri C, Bougault CM, VanNieuwenhze MS, Grangeasse C, Simorre JP Nat Commun. 2016 Jun 27;7:12071. doi: 10.1038/ncomms12071. PMID:27346279^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fleurie A, Lesterlin C, Manuse S, Zhao C, Cluzel C, Lavergne JP, Franz-Wachtel M, Macek B, Combet C, Kuru E, VanNieuwenhze MS, Brun YV, Sherratt D, Grangeasse C. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Nature. 2014 Dec 11;516(7530):259-62. doi: 10.1038/nature13966. Epub 2014 Nov 26. PMID:25470041 doi:https://dx.doi.org/10.1038/nature13966
↑ Holeckova N, Doubravova L, Massidda O, Molle V, Buriankova K, Benada O, Kofronova O, Ulrych A, Branny P. LocZ is a new cell division protein involved in proper septum placement in Streptococcus pneumoniae. MBio. 2014 Dec 30;6(1):e01700-14. doi: 10.1128/mBio.01700-14. PMID:25550321 doi:https://dx.doi.org/10.1128/mBio.01700-14
↑ Manuse S, Jean NL, Guinot M, Lavergne JP, Laguri C, Bougault CM, VanNieuwenhze MS, Grangeasse C, Simorre JP. Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ. Nat Commun. 2016 Jun 27;7:12071. doi: 10.1038/ncomms12071. PMID:27346279 doi:https://dx.doi.org/10.1038/ncomms12071

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Fleurie A, Lesterlin C, Manuse S, Zhao C, Cluzel C, Lavergne JP, Franz-Wachtel M, Macek B, Combet C, Kuru E, VanNieuwenhze MS, Brun YV, Sherratt D, Grangeasse C. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Nature. 2014 Dec 11;516(7530):259-62. doi: 10.1038/nature13966. Epub 2014 Nov 26. PMID:25470041 doi:https://dx.doi.org/10.1038/nature13966

[2] Holeckova N, Doubravova L, Massidda O, Molle V, Buriankova K, Benada O, Kofronova O, Ulrych A, Branny P. LocZ is a new cell division protein involved in proper septum placement in Streptococcus pneumoniae. MBio. 2014 Dec 30;6(1):e01700-14. doi: 10.1128/mBio.01700-14. PMID:25550321 doi:https://dx.doi.org/10.1128/mBio.01700-14

[3] Manuse S, Jean NL, Guinot M, Lavergne JP, Laguri C, Bougault CM, VanNieuwenhze MS, Grangeasse C, Simorre JP. Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ. Nat Commun. 2016 Jun 27;7:12071. doi: 10.1038/ncomms12071. PMID:27346279 doi:https://dx.doi.org/10.1038/ncomms12071

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==Solution structure of MapZ extracellular domain first subdomain==
-<StructureSection load='2nd9' size='340' side='right'caption='[[2nd9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2nd9' size='340' side='right'caption='[[2nd9]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2nd9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strr6 Strr6]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ND9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ND9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2nd9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ND9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ND9 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2nda|2nda]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nd9 OCA], [https://pdbe.org/2nd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nd9 RCSB], [https://www.ebi.ac.uk/pdbsum/2nd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nd9 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mapZ, spr0334 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nd9 OCA], [https://pdbe.org/2nd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nd9 RCSB], [https://www.ebi.ac.uk/pdbsum/2nd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nd9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/MAPZ_STRR6 MAPZ_STRR6]] Early cell division protein that marks the future cell division site and supports proper FtsZ ring positioning.[HAMAP-Rule:MF_01941]<ref>PMID:25470041</ref> <ref>PMID:25550321</ref>
+[https://www.uniprot.org/uniprot/MAPZ_STRR6 MAPZ_STRR6] Early cell division protein that marks the future cell division site and supports proper FtsZ ring positioning.[HAMAP-Rule:MF_01941]<ref>PMID:25470041</ref> <ref>PMID:25550321</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Strr6]]
+[[Category: Streptococcus pneumoniae R6]]
-[[Category: Bougault, C M]]
+[[Category: Bougault CM]]
-[[Category: Grangeasse, C]]
+[[Category: Grangeasse C]]
-[[Category: Guinot, M]]
+[[Category: Guinot M]]
-[[Category: Jean, N L]]
+[[Category: Jean NL]]
-[[Category: Manuse, S]]
+[[Category: Manuse S]]
-[[Category: Simorre, J P]]
+[[Category: Simorre J-P]]
-[[Category: Cell cycle]]
-[[Category: Division]]
-[[Category: Ftsz]]
-[[Category: Mapz]]
-[[Category: Peptidoglycan]]

2nd9: Difference between revisions

Revision as of 10:27, 15 March 2023

Solution structure of MapZ extracellular domain first subdomain

Structural highlights

Function

Publication Abstract from PubMed

References

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