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| <StructureSection load='7a02' size='340' side='right'caption='[[7a02]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='7a02' size='340' side='right'caption='[[7a02]], [[Resolution|resolution]] 3.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7a02]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A02 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A02 FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B1995_14460, B2J90_04405, BACERE00183_02460, CNQ78_18995 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ATCC 14579])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a02 OCA], [https://pdbe.org/7a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a02 RCSB], [https://www.ebi.ac.uk/pdbsum/7a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a02 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a02 OCA], [https://pdbe.org/7a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a02 RCSB], [https://www.ebi.ac.uk/pdbsum/7a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a02 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by beta-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores.
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| Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli.,Pradhan B, Liedtke J, Sleutel M, Lindback T, Zegeye ED, O Sullivan K, Llarena AK, Brynildsrud O, Aspholm M, Remaut H EMBO J. 2021 May 25:e106887. doi: 10.15252/embj.2020106887. PMID:34031903<ref>PMID:34031903</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7a02" style="background-color:#fffaf0;"></div>
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| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Atcc 14579]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Aspholm, M]] | | [[Category: Aspholm M]] |
| [[Category: Brynildsrud, O]] | | [[Category: Brynildsrud O]] |
| [[Category: Liedtke, J]] | | [[Category: Liedtke J]] |
| [[Category: Lindback, T]] | | [[Category: Lindback T]] |
| [[Category: Llarena, A K]] | | [[Category: Llarena AK]] |
| [[Category: Pradhan, B]] | | [[Category: Pradhan B]] |
| [[Category: Remaut, H]] | | [[Category: Remaut H]] |
| [[Category: Sleutel, M]] | | [[Category: Sleutel M]] |
| [[Category: Disulphide bridge]]
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| [[Category: Endospore]]
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| [[Category: Gram-positive]]
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| [[Category: Helical reconstruction]]
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| [[Category: Pilli]]
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| [[Category: Unknown function]]
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