7mtt

Crystal structure of colibactin self-resistance protein ClbS in complex with two molecules of CHES

spinqualitylabelsall models 7mtt, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7mtt is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: NHE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0P7K8_ECOLX Publication Abstract from PubMed The natural product colibactin, along with its associated biosynthetic gene cluster, is an example system for the role microbially derived small molecules play in the human microbiome. This is particularly relevant in the human gut, where host microbiota is involved in various disorders, including colorectal cancer pathogenesis. Bacteria harboring the colibactin gene cluster induce alkylation of nucleobases in host DNA, forming interstrand cross-links both in vivo and in vitro. These lesions can lead to deleterious double-strand breaks and have been identified as the primary mechanism of colibactin-induced cytotoxicity. The gene product ClbS is one of several mechanisms utilized by the producing bacteria to maintain genome integrity. ClbS catalyzes hydrolytic inactivation of colibactin and has been shown to bind DNA, incurring self-resistance. Presented is the molecular basis for ClbS bound to a DNA oligonucleotide. The structure shows the interaction of the protein with the ends of a DNA duplex with terminal nucleotides flipped to the enzyme active site. The structure suggests an additional function for ClbS, the binding to damaged DNA followed by repair. Additionally, our study provides general insight into the function of the widely distributed and largely uncharacterized DUF1706 protein family. Structural Basis for the Interactions of the Colibactin Resistance Gene Product ClbS with DNA.,Tripathi P, Bruner SD Biochemistry. 2021 May 25;60(20):1619-1625. doi: 10.1021/acs.biochem.1c00201., Epub 2021 May 4. PMID:33945270 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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