1b3a: Difference between revisions

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[[Image:1b3a.gif|left|200px]]<br />

[[Image:1b3a.gif|left|200px]]<br /><applet load="1b3a" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1b3a" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1b3a, resolution 1.6Å" />

'''TOTAL CHEMICAL SYNTHESIS AND HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE POTENT ANTI-HIV PROTEIN AOP-RANTES'''<br />

==Overview==

BACKGROUND: RANTES is a CC-type chemokine protein that acts as a, chemoattractant for several kinds of leukocytes, playing an important, pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1), into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and, inhibits HIV-1 entry into peripheral blood cells. Interaction with, chemokine receptors involves a distinct set of residues at the amino, terminus of RANTES. This finding was utilized in the development of a, chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that, was originally produced from the recombinant protein using semisynthetic, methods. RESULTS: AOP-RANTES has been produced by a novel total chemical, synthesis that provides efficient, direct access to large amounts of this, anti-HIV protein analog. The crystal structure of chemically synthesized, AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is, a dimer, with the amino-terminal pentane oxime moiety clearly defined., CONCLUSIONS: Total chemical synthesis of AOP-RANTES provides a convenient, method of producing the multi-milligram quantities of this protein needed, to investigate the molecular basis of receptor binding and antiviral, activity. This work provides the first truly high-resolution structure of, a RANTES protein, although the structure of RANTES was known from previous, nuclear magnetic resonance (NMR) determinations.

BACKGROUND: RANTES is a CC-type chemokine protein that acts as a chemoattractant for several kinds of leukocytes, playing an important pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1) into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and inhibits HIV-1 entry into peripheral blood cells. Interaction with chemokine receptors involves a distinct set of residues at the amino terminus of RANTES. This finding was utilized in the development of a chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that was originally produced from the recombinant protein using semisynthetic methods. RESULTS: AOP-RANTES has been produced by a novel total chemical synthesis that provides efficient, direct access to large amounts of this anti-HIV protein analog. The crystal structure of chemically synthesized AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is a dimer, with the amino-terminal pentane oxime moiety clearly defined. CONCLUSIONS: Total chemical synthesis of AOP-RANTES provides a convenient method of producing the multi-milligram quantities of this protein needed to investigate the molecular basis of receptor binding and antiviral activity. This work provides the first truly high-resolution structure of a RANTES protein, although the structure of RANTES was known from previous nuclear magnetic resonance (NMR) determinations.

==Disease==

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==About this Structure==

1B3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 and AOP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1B3A OCA].

1B3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=AOP:'>AOP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3A OCA].

==Reference==

Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES., Wilken J, Hoover D, Thompson DA, Barlow PN, McSparron H, Picard L, Wlodawer A, Lubkowski J, Kent SB, Chem Biol. 1999 Jan;6(1):43-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9889151 9889151]

[[Category: Single protein]]

[[Category: Barlow, P.N.]]

[[Category: Barlow, P N.]]

[[Category: Hoover, D.]]

[[Category: Kent, S.B.H.]]

[[Category: Kent, S B.H.]]

[[Category: Lubkowski, J.]]

[[Category: Mcsparron, H.]]

[[Category: Picard, L.]]

[[Category: Thompson, D.A.]]

[[Category: Thompson, D A.]]

[[Category: Wilken, J.]]

[[Category: Wlodawer, A.]]

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[[Category: rantes]]

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-[[Image:1b3a.gif|left|200px]]<br />
+[[Image:1b3a.gif|left|200px]]<br /><applet load="1b3a" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1b3a" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1b3a, resolution 1.6&Aring;" />
 '''TOTAL CHEMICAL SYNTHESIS AND HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE POTENT ANTI-HIV PROTEIN AOP-RANTES'''<br />
 ==Overview==
-BACKGROUND: RANTES is a CC-type chemokine protein that acts as a, chemoattractant for several kinds of leukocytes, playing an important, pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1), into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and, inhibits HIV-1 entry into peripheral blood cells. Interaction with, chemokine receptors involves a distinct set of residues at the amino, terminus of RANTES. This finding was utilized in the development of a, chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that, was originally produced from the recombinant protein using semisynthetic, methods. RESULTS: AOP-RANTES has been produced by a novel total chemical, synthesis that provides efficient, direct access to large amounts of this, anti-HIV protein analog. The crystal structure of chemically synthesized, AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is, a dimer, with the amino-terminal pentane oxime moiety clearly defined., CONCLUSIONS: Total chemical synthesis of AOP-RANTES provides a convenient, method of producing the multi-milligram quantities of this protein needed, to investigate the molecular basis of receptor binding and antiviral, activity. This work provides the first truly high-resolution structure of, a RANTES protein, although the structure of RANTES was known from previous, nuclear magnetic resonance (NMR) determinations.
+BACKGROUND: RANTES is a CC-type chemokine protein that acts as a chemoattractant for several kinds of leukocytes, playing an important pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1) into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and inhibits HIV-1 entry into peripheral blood cells. Interaction with chemokine receptors involves a distinct set of residues at the amino terminus of RANTES. This finding was utilized in the development of a chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that was originally produced from the recombinant protein using semisynthetic methods. RESULTS: AOP-RANTES has been produced by a novel total chemical synthesis that provides efficient, direct access to large amounts of this anti-HIV protein analog. The crystal structure of chemically synthesized AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is a dimer, with the amino-terminal pentane oxime moiety clearly defined. CONCLUSIONS: Total chemical synthesis of AOP-RANTES provides a convenient method of producing the multi-milligram quantities of this protein needed to investigate the molecular basis of receptor binding and antiviral activity. This work provides the first truly high-resolution structure of a RANTES protein, although the structure of RANTES was known from previous nuclear magnetic resonance (NMR) determinations.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-B3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 and AOP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B3A OCA].
+B3A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=AOP:'>AOP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3A OCA].
 ==Reference==
 Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES., Wilken J, Hoover D, Thompson DA, Barlow PN, McSparron H, Picard L, Wlodawer A, Lubkowski J, Kent SB, Chem Biol. 1999 Jan;6(1):43-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9889151 9889151]
 [[Category: Single protein]]
-[[Category: Barlow, P.N.]]
+[[Category: Barlow, P N.]]
 [[Category: Hoover, D.]]
-[[Category: Kent, S.B.H.]]
+[[Category: Kent, S B.H.]]
 [[Category: Lubkowski, J.]]
 [[Category: Mcsparron, H.]]
 [[Category: Picard, L.]]
-[[Category: Thompson, D.A.]]
+[[Category: Thompson, D A.]]
 [[Category: Wilken, J.]]
 [[Category: Wlodawer, A.]]
@@ Line 33: / Line 32: @@
 [[Category: rantes]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:05:09 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:51:03 2008''