7jqp: Difference between revisions
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==The Phi-28 gp11 DNA packaging Motor== | ==The Phi-28 gp11 DNA packaging Motor== | ||
<StructureSection load='7jqp' size='340' side='right'caption='[[7jqp]]' scene=''> | <StructureSection load='7jqp' size='340' side='right'caption='[[7jqp]], [[Resolution|resolution]] 2.89Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQP FirstGlance]. <br> | <table><tr><td colspan='2'>[[7jqp]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_phage_asccphi28 Lactococcus lactis phage asccphi28]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JQP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqp OCA], [https://pdbe.org/7jqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqp RCSB], [https://www.ebi.ac.uk/pdbsum/7jqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqp ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7jq6|7jq6]], [[7jq7|7jq7]]</div></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jqp OCA], [https://pdbe.org/7jqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jqp RCSB], [https://www.ebi.ac.uk/pdbsum/7jqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jqp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Double-stranded DNA viruses package their genomes into pre-assembled capsids using virally-encoded ASCE ATPase ring motors. We present the first atomic-resolution crystal structure of a multimeric ring form of a viral dsDNA packaging motor, the ATPase of the asccphi28 phage, and characterize its atomic-level dynamics via long timescale molecular dynamics simulations. Based on these results, and previous single-molecule data and cryo-EM reconstruction of the homologous phi29 motor, we propose an overall packaging model that is driven by helical-to-planar transitions of the ring motor. These transitions are coordinated by inter-subunit interactions that regulate catalytic and force-generating events. Stepwise ATP binding to individual subunits increase their affinity for the helical DNA phosphate backbone, resulting in distortion away from the planar ring towards a helical configuration, inducing mechanical strain. Subsequent sequential hydrolysis events alleviate the accumulated mechanical strain, allowing a stepwise return of the motor to the planar conformation, translocating DNA in the process. This type of helical-to-planar mechanism could serve as a general framework for ring ATPases. | |||
Atomistic basis of force generation, translocation, and coordination in a viral genome packaging motor.,Pajak J, Dill E, Reyes-Aldrete E, White MA, Kelch BA, Jardine PJ, Arya G, Morais MC Nucleic Acids Res. 2021 May 29. pii: 6288440. doi: 10.1093/nar/gkab372. PMID:34050764<ref>PMID:34050764</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7jqp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Lactococcus lactis phage asccphi28]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Dill E]] | [[Category: Dill, E]] | ||
[[Category: Morais | [[Category: Morais, M C]] | ||
[[Category: White | [[Category: White, M A]] | ||
[[Category: Asce fold]] | |||
[[Category: Atpase]] | |||
[[Category: Dna packaging]] | |||
[[Category: Motor]] | |||
[[Category: Phage]] | |||
[[Category: Viral protein]] | |||
