6zm8: Difference between revisions
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==Structure of muramidase from Acremonium alcalophilum== | ==Structure of muramidase from Acremonium alcalophilum== | ||
<StructureSection load='6zm8' size='340' side='right'caption='[[6zm8]]' scene=''> | <StructureSection load='6zm8' size='340' side='right'caption='[[6zm8]], [[Resolution|resolution]] 0.78Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZM8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6zm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acremonium_alcalophilum Acremonium alcalophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZM8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zm8 OCA], [https://pdbe.org/6zm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zm8 RCSB], [https://www.ebi.ac.uk/pdbsum/6zm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zm8 ProSAT]</span></td></tr> | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zm8 OCA], [https://pdbe.org/6zm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zm8 RCSB], [https://www.ebi.ac.uk/pdbsum/6zm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zm8 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall. They are found in many of the glycoside hydrolase (GH) families. Family GH25 contains muramidases/lysozymes, known as CH type lysozymes, as they were initially discovered in the Chalaropsis species of fungus. The characterized enzymes from GH25 exhibit both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetylmuramidase activities, cleaving the beta-1,4-glycosidic bond between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) moieties in the carbohydrate backbone of bacterial peptidoglycan. Here, a set of fungal GH25 muramidases were identified from a sequence search, cloned and expressed and screened for their ability to digest bacterial peptidoglycan, to be used in a commercial application in chicken feed. The screen identified the enzyme from Acremonium alcalophilum JCM 736 as a suitable candidate for this purpose and its relevant biochemical and biophysical and properties are described. We report the crystal structure of the A. alcalophilum enzyme at atomic, 0.78 A resolution, together with that of its homologue from Trichobolus zukalii at 1.4 A, and compare these with the structures of homologues. GH25 enzymes offer a new solution in animal feed applications such as for processing bacterial debris in the animal gut. | |||
Fungal GH25 muramidases: New family members with applications in animal nutrition and a crystal structure at 0.78A resolution.,Moroz OV, Blagova E, Taylor E, Turkenburg JP, Skov LK, Gippert GP, Schnorr KM, Ming L, Ye L, Klausen M, Cohn MT, Schmidt EGW, Nymand-Grarup S, Davies GJ, Wilson KS PLoS One. 2021 Mar 12;16(3):e0248190. doi: 10.1371/journal.pone.0248190., eCollection 2021. PMID:33711051<ref>PMID:33711051</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6zm8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Acremonium alcalophilum]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Blagova E]] | [[Category: Lysozyme]] | ||
[[Category: Cohn | [[Category: Blagova, E]] | ||
[[Category: Davies | [[Category: Cohn, M T]] | ||
[[Category: Gippert | [[Category: Davies, G J]] | ||
[[Category: Klausen M]] | [[Category: Gippert, G P]] | ||
[[Category: Ming L]] | [[Category: Klausen, M]] | ||
[[Category: Moroz | [[Category: Ming, L]] | ||
[[Category: Nymand-Grarup S]] | [[Category: Moroz, O V]] | ||
[[Category: Schmidt | [[Category: Nymand-Grarup, S]] | ||
[[Category: Schnorr | [[Category: Schmidt, E G.W]] | ||
[[Category: Skov | [[Category: Schnorr, K M]] | ||
[[Category: Taylor E]] | [[Category: Skov, L K]] | ||
[[Category: Turkenburg | [[Category: Taylor, E]] | ||
[[Category: Wilson | [[Category: Turkenburg, J P]] | ||
[[Category: Ye L]] | [[Category: Wilson, K S]] | ||
[[Category: Ye, L]] | |||
[[Category: Fungal]] | |||
[[Category: Gh25]] | |||
[[Category: Hydrolase]] | |||
[[Category: Industrial application]] | |||
[[Category: Muramidase]] | |||
[[Category: Peptidoglycan cleavage]] | |||
Revision as of 07:06, 27 January 2022
Structure of muramidase from Acremonium alcalophilum
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