1f08: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1f08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F08 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1f08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F08 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f08 OCA], [https://pdbe.org/1f08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f08 RCSB], [https://www.ebi.ac.uk/pdbsum/1f08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f08 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f08 OCA], [https://pdbe.org/1f08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f08 RCSB], [https://www.ebi.ac.uk/pdbsum/1f08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f08 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/VE1_BPV1 VE1_BPV1]] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.  
[https://www.uniprot.org/uniprot/VE1_BPV1 VE1_BPV1] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f08 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f08 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure of the E1 DNA binding domain refined to 1.9 A resolution. Residues critical for DNA binding are located on an extended loop and an alpha helix. We identify the E1 dimerization surface by selective mutations at an E1/E1 interface observed in the crystal and propose a model for the (E1)2-DNA complex. These and other observations suggest how the E1 DNA binding domain orchestrates assembly of the hexameric helicase on the ori.
Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus.,Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L Mol Cell. 2000 Jul;6(1):149-58. PMID:10949036<ref>PMID:10949036</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1f08" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Replication protein E1|Replication protein E1]]
*[[Replication protein E1|Replication protein E1]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bovine papillomavirus]]
[[Category: Bovine papillomavirus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chen, G]]
[[Category: Chen G]]
[[Category: Enemark, E J]]
[[Category: Enemark EJ]]
[[Category: Joshua-Tor, L]]
[[Category: Joshua-Tor L]]
[[Category: Stenlund, A]]
[[Category: Stenlund A]]
[[Category: Vaughn, D E]]
[[Category: Vaughn DE]]
[[Category: Dna-binding domain]]
[[Category: Helicase]]
[[Category: Initiator protein]]
[[Category: Papillomavirus]]
[[Category: Replication]]

Latest revision as of 07:09, 7 February 2024

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS

1f08, resolution 1.90Å

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