1f7c: Difference between revisions

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<StructureSection load='1f7c' size='340' side='right'caption='[[1f7c]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='1f7c' size='340' side='right'caption='[[1f7c]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1f7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F7C FirstGlance]. <br>
<table><tr><td colspan='2'>[[1f7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F7C FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f7c OCA], [https://pdbe.org/1f7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f7c RCSB], [https://www.ebi.ac.uk/pdbsum/1f7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f7c ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f7c OCA], [https://pdbe.org/1f7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f7c RCSB], [https://www.ebi.ac.uk/pdbsum/1f7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f7c ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RHG26_CHICK RHG26_CHICK]] GTPase-activating protein for RHOA and CDC42.<ref>PMID:8649427</ref> <ref>PMID:10982819</ref>
[https://www.uniprot.org/uniprot/RHG26_CHICK RHG26_CHICK] GTPase-activating protein for RHOA and CDC42.<ref>PMID:8649427</ref> <ref>PMID:10982819</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f7c ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f7c ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cellular signaling by small G-proteins is down-regulated by GTPase-activating proteins (GAPs), which increase the rate of GTP hydrolysis. The GTPase regulator associated with focal adhesion kinase (Graf) exhibits GAP activity toward the RhoA and Cdc42 GTPases, but is only weakly active toward the closely related Rac1. We determined the crystal structure of a 231-residue fragment of Graf (GrafGAP), a domain containing the GAP activity, at 2.4-A resolution. The structure clarifies the boundaries of the functional domain and yields insight to the mechanism of substrate recognition. Modeling its interaction with substrate suggested that a favorable interaction with Glu-95 of Cdc42 (Glu-97 of RhoA) would be absent with the corresponding Ala-95 of Rac1. Indeed, GrafGAP activity is diminished approximately 40-fold toward a Cdc42 E95A mutant, whereas a approximately 10-fold increase is observed for a Rac1 A95E mutant. The GrafGAP epitope that apparently interacts with Glu-95(Glu-97) contains Asn-225, which was recently found mutated in some myeloid leukemia patients. We conclude that position 95 of the GTPase is an important determinant for GrafGAP specificity in cellular function and tumor suppression.
Structure of the BH domain from graf and its implications for Rho GTPase recognition.,Longenecker KL, Zhang B, Derewenda U, Sheffield PJ, Dauter Z, Parsons JT, Zheng Y, Derewenda ZS J Biol Chem. 2000 Dec 8;275(49):38605-10. PMID:10982819<ref>PMID:10982819</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1f7c" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chick]]
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Derewenda, U]]
[[Category: Derewenda U]]
[[Category: Derewenda, Z S]]
[[Category: Derewenda ZS]]
[[Category: Longenecker, K L]]
[[Category: Longenecker KL]]
[[Category: Sheffield, P J]]
[[Category: Sheffield PJ]]
[[Category: Zheng, Y]]
[[Category: Zheng Y]]
[[Category: Bh domain]]
[[Category: Gap]]
[[Category: Gtpase activating protein]]
[[Category: Rho gtpase regulator]]
[[Category: Signaling protein]]

Latest revision as of 07:11, 7 February 2024

CRYSTAL STRUCTURE OF THE BH DOMAIN FROM GRAF, THE GTPASE REGULATOR ASSOCIATED WITH FOCAL ADHESION KINASE

1f7c, resolution 2.40Å

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