7ohf: Difference between revisions
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==Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels== | ==Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels== | ||
<StructureSection load='7ohf' size='340' side='right'caption='[[7ohf]]' scene=''> | <StructureSection load='7ohf' size='340' side='right'caption='[[7ohf]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHF FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ohf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_com1 Pyrococcus furiosus com1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHF FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohf OCA], [https://pdbe.org/7ohf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohf RCSB], [https://www.ebi.ac.uk/pdbsum/7ohf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohf ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFC_02870 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1185654 Pyrococcus furiosus COM1])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohf OCA], [https://pdbe.org/7ohf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohf RCSB], [https://www.ebi.ac.uk/pdbsum/7ohf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohf ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier for routine high-resolution imaging and limits the current throughput of the technique. We show that a nanofluidic sample support with well-defined geometry can be used to prepare cryo-EM specimens with reproducible ice thickness from picoliter sample volumes. The sample solution is contained in electron-transparent nanochannels that provide uniform thickness gradients without further optimisation and eliminate the potentially destructive air-water interface. We demonstrate the possibility to perform high-resolution structure determination with three standard protein specimens. Nanofabricated sample supports bear potential to automate the cryo-EM workflow, and to explore new frontiers for cryo-EM applications such as time-resolved imaging and high-throughput screening. | |||
Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes.,Huber ST, Sarajlic E, Huijink R, Weis F, Evers WH, Jakobi AJ Elife. 2022 Jan 21;11. pii: 72629. doi: 10.7554/eLife.72629. PMID:35060902<ref>PMID:35060902</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ohf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Evers | [[Category: Pyrococcus furiosus com1]] | ||
[[Category: Huber | [[Category: Evers, W H]] | ||
[[Category: Huijink R]] | [[Category: Huber, S T]] | ||
[[Category: Jakobi | [[Category: Huijink, R]] | ||
[[Category: Sarajlic E]] | [[Category: Jakobi, A J]] | ||
[[Category: Sarajlic, E]] | |||
[[Category: Iron storage]] | |||
[[Category: Metal transport]] | |||
