1f3s: Difference between revisions

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[[Image:1f3s.~~gif~~|left|200px]]

[[Image:1f3s.png|left|200px]]

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~~'''~~SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.~~'''~~

===SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.===

~~==Overview==~~

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~~We have designed a DNA sequence~~, ~~d(G-G-G-T-T-C-A-G-G)~~, ~~which dimerizes~~ to ~~form a 2-fold symmetric G-quadruplex in which G~~(~~syn)~~. ~~G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G~~. ~~(C-A~~) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,~~(15)N)-labeled sample of~~ the ~~d(G-G-G-T-T-C-A-G-G) sequence~~. ~~The solution structure establishes that the guanine base~~-~~pairs with the cytosine through Watson~~-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.

The line below this paragraph, {{ABSTRACT_PUBMED_10926497}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 10926497 is the PubMed ID number.

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==About this Structure==

Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].

Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].

==Reference==

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[[Category: Triad dna model]]

[[Category: Uniform 13c,15n-labeled dna]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 15~~:51:27 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:37:55 2008''

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-[[Image:1f3s.gif|left|200px]]
+{{Seed}}
+[[Image:1f3s.png|left|200px]]
 <!--
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 {{STRUCTURE_1f3s|  PDB=1f3s  |  SCENE=  }}
-'''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''
+===SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.===
-==Overview==
+<!--
-We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
+The line below this paragraph, {{ABSTRACT_PUBMED_10926497}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10926497 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10926497}}
 ==About this Structure==
-Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].
+Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].
 ==Reference==
@@ Line 29: / Line 33: @@
 [[Category: Triad dna model]]
 [[Category: Uniform 13c,15n-labeled dna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:51:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 02:37:55 2008''