1mj0: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1mj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Designed_synthetic_gene Designed synthetic gene]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJ0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1mj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Designed_synthetic_gene Designed synthetic gene]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJ0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.031&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mj0 OCA], [https://pdbe.org/1mj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mj0 RCSB], [https://www.ebi.ac.uk/pdbsum/1mj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mj0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mj0 OCA], [https://pdbe.org/1mj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mj0 RCSB], [https://www.ebi.ac.uk/pdbsum/1mj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mj0 ProSAT]</span></td></tr>
</table>
</table>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mj0 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mj0 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ankyrin repeat (AR) proteins mediate innumerable protein-protein interactions in virtually all phyla. This finding suggested the use of AR proteins as designed binding molecules. Based on sequence and structural analyses, we designed a consensus AR with fixed framework and randomized interacting residues. We generated several combinatorial libraries of AR proteins consisting of defined numbers of this repeat. Randomly chosen library members are expressed in soluble form in the cytoplasm of Escherichia coli constituting up to 30% of total cellular protein and show high thermodynamic stability. We determined the crystal structure of one of those library members to 2.0-A resolution, providing insight into the consensus AR fold. Besides the highly complementary hydrophobic repeat-repeat interfaces and the absence of structural irregularities in the consensus AR protein, the regular and extended hydrogen bond networks in the beta-turn and loop regions are noteworthy. Furthermore, all residues found in the turn region of the Ramachandran plot are glycines. Many of these features also occur in natural AR proteins, but not in this rigorous and standardized fashion. We conclude that the AR domain fold is an intrinsically very stable and well-expressed scaffold, able to display randomized interacting residues. This scaffold represents an excellent basis for the design of novel binding molecules.
Designed to be stable: crystal structure of a consensus ankyrin repeat protein.,Kohl A, Binz HK, Forrer P, Stumpp MT, Pluckthun A, Grutter MG Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1700-5. Epub 2003 Feb 3. PMID:12566564<ref>PMID:12566564</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1mj0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Designed synthetic gene]]
[[Category: Designed synthetic gene]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Binz, H K]]
[[Category: Binz HK]]
[[Category: Forrer, P]]
[[Category: Forrer P]]
[[Category: Gruetter, M G]]
[[Category: Gruetter MG]]
[[Category: Kohl, A]]
[[Category: Kohl A]]
[[Category: Plueckthun, A]]
[[Category: Plueckthun A]]
[[Category: Stumpp, M T]]
[[Category: Stumpp MT]]
[[Category: Ankyrin repeat]]
[[Category: Consensus]]
[[Category: De novo protein]]
[[Category: Protein engineering]]
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