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<StructureSection load='1ngm' size='340' side='right'caption='[[1ngm]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
<StructureSection load='1ngm' size='340' side='right'caption='[[1ngm]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1ngm]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGM FirstGlance]. <br>
<table><tr><td colspan='2'>[[1ngm]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGM FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngm OCA], [https://pdbe.org/1ngm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngm RCSB], [https://www.ebi.ac.uk/pdbsum/1ngm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngm ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngm OCA], [https://pdbe.org/1ngm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngm RCSB], [https://www.ebi.ac.uk/pdbsum/1ngm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngm ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/TF3B_YEAST TF3B_YEAST]] General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC. [[https://www.uniprot.org/uniprot/TBP_YEAST TBP_YEAST]] General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.<ref>PMID:9618449</ref> <ref>PMID:12138208</ref> <ref>PMID:12516863</ref>
[https://www.uniprot.org/uniprot/TBP_YEAST TBP_YEAST] General transcription factor that functions at the core of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.<ref>PMID:9618449</ref> <ref>PMID:12138208</ref> <ref>PMID:12516863</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngm ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngm ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transcription factor IIIB (TFIIIB), consisting of the TATA-binding protein (TBP), TFIIB-related factor (Brf1) and Bdp1, is a central component in basal and regulated transcription by RNA polymerase III. TFIIIB recruits its polymerase to the promoter and subsequently has an essential role in the formation of the open initiation complex. The amino-terminal half of Brf1 shares a high degree of sequence similarity with the polymerase II general transcription factor TFIIB, but it is the carboxy-terminal half of Brf1 that contributes most of its binding affinity with TBP. The principal anchoring region is located between residues 435 and 545 of yeast Brf1, comprising its homology domain II. The same region also provides the primary interface for assembling Bdp1 into the TFIIIB complex. We report here a 2.95 A resolution crystal structure of the ternary complex containing Brf1 homology domain II, the conserved region of TBP and 19 base pairs of U6 promoter DNA. The structure reveals the core interface for assembly of TFIIIB and demonstrates how the loosely packed Brf1 domain achieves remarkable binding specificity with the convex and lateral surfaces of TBP.
Crystal structure of a transcription factor IIIB core interface ternary complex.,Juo ZS, Kassavetis GA, Wang J, Geiduschek EP, Sigler PB Nature. 2003 Apr 3;422(6931):534-9. Epub 2003 Mar 26. PMID:12660736<ref>PMID:12660736</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ngm" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Geiduschek, E P]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Juo, Z S]]
[[Category: Geiduschek EP]]
[[Category: Kassavetis, G A]]
[[Category: Juo ZS]]
[[Category: Sigler, P B]]
[[Category: Kassavetis GA]]
[[Category: Wang, J]]
[[Category: Sigler PB]]
[[Category: Brf]]
[[Category: Wang J]]
[[Category: Brf1]]
[[Category: Protein-dna complex]]
[[Category: Taf]]
[[Category: Tbp]]
[[Category: Tfiiib]]
[[Category: Transcription]]
[[Category: Transcription-dna complex]]

Latest revision as of 07:56, 14 February 2024

Crystal structure of a yeast Brf1-TBP-DNA ternary complex

1ngm, resolution 2.95Å

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