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<StructureSection load='1toc' size='340' side='right'caption='[[1toc]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='1toc' size='340' side='right'caption='[[1toc]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1toc]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Argasid_tick Argasid tick] and [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TOC FirstGlance]. <br>
<table><tr><td colspan='2'>[[1toc]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Ornithodoros_moubata Ornithodoros moubata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TOC FirstGlance]. <br>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1toc OCA], [https://pdbe.org/1toc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1toc RCSB], [https://www.ebi.ac.uk/pdbsum/1toc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1toc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1toc OCA], [https://pdbe.org/1toc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1toc RCSB], [https://www.ebi.ac.uk/pdbsum/1toc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1toc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/THRB_BOVIN THRB_BOVIN]] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity). [[https://www.uniprot.org/uniprot/ORNT_ORNMO ORNT_ORNMO]] Potent (Ki = 10(-12) M) and highly selective thrombin inhibitor.  
[https://www.uniprot.org/uniprot/THRB_BOVIN THRB_BOVIN] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1toc ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1toc ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ornithodorin, isolated from the blood sucking soft tick Ornithodoros moubata, is a potent (Ki = 10(-12) M) and highly selective thrombin inhibitor. Internal sequence homology indicates a two domain protein. Each domain resembles the Kunitz inhibitor basic pancreatic trypsin inhibitor (BPTI) and also the tick anticoagulant peptide (TAP) isolated from the same organism. The 3.1 A crystal structure of the ornithodorin-thrombin complex confirms that both domains of ornithodorin exhibit a distorted BPTI-like fold. The N-terminal portion and the C-terminal helix of each domain are structurally very similar to BPTI, whereas the regions corresponding to the binding loop of BPTI adopt different conformations. Neither of the two 'reactive site loops' of ornithodorin contacts the protease in the ornithodorin-thrombin complex. Instead, the N-terminal residues of ornithodorin bind to the active site of thrombin, reminiscent of the thrombin-hirudin interaction. The C-terminal domain binds at the fibrinogen recognition exosite. Molecular recognition of its target protease by this double-headed Kunitz-type inhibitor diverges considerably from other members of this intensely studied superfamily. The complex structure provides a model to explain the perplexing results of mutagenesis studies on the TAP-factor Xa interaction.
The ornithodorin-thrombin crystal structure, a key to the TAP enigma?,van de Locht A, Stubbs MT, Bode W, Friedrich T, Bollschweiler C, Hoffken W, Huber R EMBO J. 1996 Nov 15;15(22):6011-7. PMID:8947023<ref>PMID:8947023</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1toc" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Proteinase 3D structures|Proteinase 3D structures]]
*[[Thrombin 3D Structures|Thrombin 3D Structures]]
*[[Thrombin 3D Structures|Thrombin 3D Structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Argasid tick]]
[[Category: Bos taurus]]
[[Category: Bovin]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Thrombin]]
[[Category: Ornithodoros moubata]]
[[Category: Bode, W]]
[[Category: Bode W]]
[[Category: Huber, R]]
[[Category: Huber R]]
[[Category: Locht, A Van De]]
[[Category: Van De Locht A]]
[[Category: Acute phase]]
[[Category: Gamma-carboxyglutamic acid]]
[[Category: Hydrolase]]
[[Category: Kringle]]
[[Category: Liver]]
[[Category: Serine protease kunitz-like inhibitor]]
[[Category: Vitamin k]]
[[Category: Zymogen]]

Revision as of 08:42, 1 May 2024

STRUCTURE OF SERINE PROTEINASE

1toc, resolution 3.10Å

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