1fce: Difference between revisions

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[[Image:1fce.jpg|left|200px]]
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{{STRUCTURE_1fce|  PDB=1fce  |  SCENE=  }}  
{{STRUCTURE_1fce|  PDB=1fce  |  SCENE=  }}  


'''PROCESSIVE ENDOCELLULASE CELF OF CLOSTRIDIUM CELLULOLYTICUM'''
===PROCESSIVE ENDOCELLULASE CELF OF CLOSTRIDIUM CELLULOLYTICUM===




==Overview==
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The mesophilic bacterium Clostridium cellulolyticum exports multienzyme complexes called cellulosomes to digest cellulose. One of the three major components of the cellulosome is the processive endocellulase CelF. The crystal structure of the catalytic domain of CelF in complex with two molecules of a thiooligosaccharide inhibitor was determined at 2.0 A resolution. This is the first three-dimensional structure to be solved of a member of the family 48 glycosyl hydrolases. The structure consists of an (alpha alpha)6-helix barrel with long loops on the N-terminal side of the inner helices, which form a tunnel, and an open cleft region covering one side of the barrel. One inhibitor molecule is enclosed in the tunnel, the other exposed in the open cleft. The active centre is located in a depression at the junction of the cleft and tunnel regions. Glu55 is the proposed proton donor in the cleavage reaction, while the corresponding base is proposed to be either Glu44 or Asp230. The orientation of the reducing ends of the inhibitor molecules together with the chain translation through the tunnel in the direction of the active centre indicates that CelF cleaves processively cellobiose from the reducing to the non-reducing end of the cellulose chain.
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{{ABSTRACT_PUBMED_9755156}}


==About this Structure==
==About this Structure==
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[[Category: Processive endo action]]
[[Category: Processive endo action]]
[[Category: Thiooligosaccharide inhibitor]]
[[Category: Thiooligosaccharide inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:02:42 2008''

Revision as of 00:02, 1 July 2008

File:1fce.png

Template:STRUCTURE 1fce

PROCESSIVE ENDOCELLULASE CELF OF CLOSTRIDIUM CELLULOLYTICUM

Template:ABSTRACT PUBMED 9755156

About this Structure

1FCE is a Single protein structure of sequence from Clostridium cellulolyticum. Full crystallographic information is available from OCA.

Reference

The crystal structure of the processive endocellulase CelF of Clostridium cellulolyticum in complex with a thiooligosaccharide inhibitor at 2.0 A resolution., Parsiegla G, Juy M, Reverbel-Leroy C, Tardif C, Belaich JP, Driguez H, Haser R, EMBO J. 1998 Oct 1;17(19):5551-62. PMID:9755156

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