Sandbox Reserved 1687: Difference between revisions

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==Function of RNA-Dependent RNA Polymerase==

RNA-dependent RNA polymerases (RdRp) are critical to the replication of RNA viruses.<ref>DOI:10.1038/s41598-018-22328-3</ref> Due to this protein's importance in the viral life cycle, they are feasible targets for vaccine development.<ref>DOI:10.1038/s41598-018-22328-3</ref> Inhibition of RdRp results in the inhibition of transcription of the viral genome, ultimately resulting in no new production of virions for the virus.

RNA-dependent RNA polymerases (RdRp) are critical to the replication and transcription of RNA viruses.<ref>DOI:10.1038/s41598-018-22328-3</ref> Due to this protein's importance in the viral life cycle, they are feasible targets for vaccine development.<ref>DOI:10.1038/s41598-018-22328-3</ref> Inhibition of RdRp results in the inhibition of transcription of the viral genome, ultimately resulting in no new production of virions for the virus.

==Structural Features of Ebola Virus RNA-Dependent RNA Polymerase[https://swissmodel.expasy.org/interactive/7EhwKr/]==

EBOV has a monomeric RNA-dependent RNA polymerase and as such, shares the characteristic <scene name='89/891377/Rh_shape/1'>right-hand shape</scene> of other monomeric RdRp composed of the fingertips, palm, and thumb subdomains. <ref>PMID:26397100</ref> The <scene name='89/891377/Fingertips_subdomain_2/4'>fingertips subdomain</scene> is composed of residues 417-439 and 489-563, the <scene name='89/891377/Palm_subdomain_1/5'>palm subdomain</scene> is composed of residues 440-488 and 563-666, and the <scene name='89/891377/Thumb_subdomain/1'>thumb</scene> is made up of residues 667-704.<ref>PMID:26397100</ref> The highly conserved motifs A-E were identified in the palm subdomain of the EBOV RdRp, but motifs F and G, which are not part of the active site, were not identified.<ref>PMID:26397100</ref>

EBOV has a monomeric RNA-dependent RNA polymerase and as such, shares the characteristic <scene name='89/891377/Rh_shape/1'>right-hand shape</scene> of other monomeric RdRp composed of the fingertips, palm, and thumb subdomains. <ref>PMID:26397100</ref> The 3D structure described here was produced by running one Zaire Ebola virus L protein sequence (Sierra Leona, Makona-G3686.1; AIE11922) on the homology modeling program, SwissModel. [https://swissmodel.expasy.org/interactive/7EhwKr/] The <scene name='89/891377/Fingertips_subdomain_2/4'>fingertips subdomain</scene> is composed of residues 417-439 and 489-563, the <scene name='89/891377/Palm_subdomain_1/5'>palm subdomain</scene> is composed of residues 440-488 and 563-666, and the <scene name='89/891377/Thumb_subdomain/1'>thumb</scene> is made up of residues 667-704.<ref>PMID:26397100</ref> The highly conserved motifs A-E were identified in the palm subdomain of the EBOV RdRp, but motifs F and G, which are not part of the active site, were not identified.<ref>PMID:26397100</ref>

==RNA Transcription and Translation==

Sandbox Reserved 1687: Difference between revisions

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 ==Function of RNA-Dependent RNA Polymerase==
-RNA-dependent RNA polymerases (RdRp) are critical to the replication of RNA viruses.<ref>DOI:10.1038/s41598-018-22328-3</ref> Due to this protein's importance in the viral life cycle, they are feasible targets for vaccine development.<ref>DOI:10.1038/s41598-018-22328-3</ref> Inhibition of RdRp results in the inhibition of transcription of the viral genome, ultimately resulting in no new production of virions for the virus.
+RNA-dependent RNA polymerases (RdRp) are critical to the replication and transcription of RNA viruses.<ref>DOI:10.1038/s41598-018-22328-3</ref> Due to this protein's importance in the viral life cycle, they are feasible targets for vaccine development.<ref>DOI:10.1038/s41598-018-22328-3</ref> Inhibition of RdRp results in the inhibition of transcription of the viral genome, ultimately resulting in no new production of virions for the virus.
 ==Structural Features of Ebola Virus RNA-Dependent RNA Polymerase[https://swissmodel.expasy.org/interactive/7EhwKr/]==
-EBOV has a monomeric RNA-dependent RNA polymerase and as such, shares the characteristic <scene name='89/891377/Rh_shape/1'>right-hand shape</scene> of other monomeric RdRp composed of the fingertips, palm, and thumb subdomains. <ref>PMID:26397100</ref> The <scene name='89/891377/Fingertips_subdomain_2/4'>fingertips subdomain</scene> is composed of residues 417-439 and 489-563, the <scene name='89/891377/Palm_subdomain_1/5'>palm subdomain</scene> is composed of residues 440-488 and 563-666, and the <scene name='89/891377/Thumb_subdomain/1'>thumb</scene> is made up of residues 667-704.<ref>PMID:26397100</ref> The highly conserved motifs A-E were identified in the palm subdomain of the EBOV RdRp, but motifs F and G, which are not part of the active site, were not identified.<ref>PMID:26397100</ref>
+EBOV has a monomeric RNA-dependent RNA polymerase and as such, shares the characteristic <scene name='89/891377/Rh_shape/1'>right-hand shape</scene> of other monomeric RdRp composed of the fingertips, palm, and thumb subdomains. <ref>PMID:26397100</ref> The 3D structure described here was produced by running one Zaire Ebola virus L protein sequence (Sierra Leona, Makona-G3686.1; AIE11922) on the homology modeling program, SwissModel. [https://swissmodel.expasy.org/interactive/7EhwKr/] The <scene name='89/891377/Fingertips_subdomain_2/4'>fingertips subdomain</scene> is composed of residues 417-439 and 489-563, the <scene name='89/891377/Palm_subdomain_1/5'>palm subdomain</scene> is composed of residues 440-488 and 563-666, and the <scene name='89/891377/Thumb_subdomain/1'>thumb</scene> is made up of residues 667-704.<ref>PMID:26397100</ref> The highly conserved motifs A-E were identified in the palm subdomain of the EBOV RdRp, but motifs F and G, which are not part of the active site, were not identified.<ref>PMID:26397100</ref>
 ==RNA Transcription and Translation==