Sandbox Reserved 1684: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Felix Valero (talk | contribs) No edit summary |
Felix Valero (talk | contribs) No edit summary |
||
| Line 39: | Line 39: | ||
Once the template strand is bound and NTP entry tunnel is formed, we can find several conserved basic residues (<scene name='89/891374/Binding_to_gtp/3'>Arg 163, Lys 167, Arg 17</scene>4) that were previously described binding the RdRd to the template strand. | Once the template strand is bound and NTP entry tunnel is formed, we can find several conserved basic residues (<scene name='89/891374/Binding_to_gtp/3'>Arg 163, Lys 167, Arg 17</scene>4) that were previously described binding the RdRd to the template strand. | ||
There is a kink at the top of the NTP entry tunnel which is stabilized by hydrogen bonding interactions involving Pro40, Glu47, and Arg49. these residues are | There is a kink at the top of the NTP entry tunnel which is stabilized by hydrogen bonding interactions involving Pro40, Glu47, and Arg49. these residues are highly conserved across picornaviruses , suggesting that the kink is common to all these viral polymerases. | ||
One side of the binding pocket for the N-terminus is almost entirely made up of glycines (Gly284, Gly285) which provide some torsional flexibility. These glycines are 100% conserved among picornaviral polymerase sequences. | One side of the binding pocket for the N-terminus is almost entirely made up of glycines (Gly284, Gly285) which provide some torsional flexibility. These glycines are 100% conserved among picornaviral polymerase sequences. | ||
There is a salt bridge between index and ring fingers that lines the NTP entry tunnel, Lys61 and Glu177 | There is a salt bridge between index and ring fingers that lines the NTP entry tunnel, Lys61 and Glu177 form this bridge and are highly conserved among picornaviruses. | ||
== References == | == References == | ||
<references/> | <references/> | ||
