1fp2: Difference between revisions

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[[Image:1fp2.~~gif~~|left|200px]]

[[Image:1fp2.png|left|200px]]

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~~'''~~CRYSTAL STRUCTURE ANALYSIS OF ISOFLAVONE O-METHYLTRANSFERASE~~'''~~

===CRYSTAL STRUCTURE ANALYSIS OF ISOFLAVONE O-METHYLTRANSFERASE===

~~==Overview==~~

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~~Chalcone O~~-~~methyltransferase (ChOMT) and isoflavone O~~-methyltransferase (IOMT) are S-adenosyl-l-methionine (SAM) dependent plant natural product methyltransferases involved in secondary metabolism in Medicago sativa (alfalfa). Here we report the crystal structure of ChOMT in complex with the product S-adenosyl-l-homocysteine and the substrate isoliquiritigenin (4,2',~~4'-trihydroxychalcone) refined~~ to ~~1.8 A as well as~~ the ~~crystal structure of IOMT in complex with the products S-adenosyl-l-homocysteine and isoformononetin~~ (~~4'-hydroxy-7-methoxyisoflavone) refined to 1~~.~~4 A~~. ~~These two OMTs constitute the first plant methyltransferases to be structurally characterized and reveal a novel oligomerization domain and the molecular determinants for substrate selection. As such~~, ~~this work provides a structural basis for understanding~~ the ~~substrate specificity of the diverse family of plant OMTs and facilitates the engineering of novel activities in this extensive class of natural product biosynthetic enzymes~~.

The line below this paragraph, {{ABSTRACT_PUBMED_11224575}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 11224575 is the PubMed ID number.

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==About this Structure==

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[[Category: Zubieta, C.]]

[[Category: Protein-product complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 16~~:35:47 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:42:58 2008''

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-[[Image:1fp2.gif|left|200px]]
+{{Seed}}
+[[Image:1fp2.png|left|200px]]
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 {{STRUCTURE_1fp2|  PDB=1fp2  |  SCENE=  }}
-'''CRYSTAL STRUCTURE ANALYSIS OF ISOFLAVONE O-METHYLTRANSFERASE'''
+===CRYSTAL STRUCTURE ANALYSIS OF ISOFLAVONE O-METHYLTRANSFERASE===
-==Overview==
+<!--
-Chalcone O-methyltransferase (ChOMT) and isoflavone O-methyltransferase (IOMT) are S-adenosyl-l-methionine (SAM) dependent plant natural product methyltransferases involved in secondary metabolism in Medicago sativa (alfalfa). Here we report the crystal structure of ChOMT in complex with the product S-adenosyl-l-homocysteine and the substrate isoliquiritigenin (4,2',4'-trihydroxychalcone) refined to 1.8 A as well as the crystal structure of IOMT in complex with the products S-adenosyl-l-homocysteine and isoformononetin (4'-hydroxy-7-methoxyisoflavone) refined to 1.4 A. These two OMTs constitute the first plant methyltransferases to be structurally characterized and reveal a novel oligomerization domain and the molecular determinants for substrate selection. As such, this work provides a structural basis for understanding the substrate specificity of the diverse family of plant OMTs and facilitates the engineering of novel activities in this extensive class of natural product biosynthetic enzymes.
+The line below this paragraph, {{ABSTRACT_PUBMED_11224575}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 11224575 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_11224575}}
 ==About this Structure==
@@ Line 26: / Line 30: @@
 [[Category: Zubieta, C.]]
 [[Category: Protein-product complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:35:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 03:42:58 2008''