7kik: Difference between revisions
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==== | ==Wheat dwarf virus Rep domain circular permutation complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+== | ||
<StructureSection load='7kik' size='340' side='right'caption='[[7kik]]' scene=''> | <StructureSection load='7kik' size='340' side='right'caption='[[7kik]], [[Resolution|resolution]] 1.73Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7kik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wheat_dwarf_virus Wheat dwarf virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIK FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kik OCA], [https://pdbe.org/7kik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kik RCSB], [https://www.ebi.ac.uk/pdbsum/7kik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kik ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kik OCA], [https://pdbe.org/7kik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kik RCSB], [https://www.ebi.ac.uk/pdbsum/7kik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kik ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/REP_WDVS REP_WDVS] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. Acts as an inhibitor of C-sense gene transcription (By similarity). | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Wheat dwarf virus]] | ||
[[Category: Gordon WR]] | |||
[[Category: Litzau LA]] | |||
[[Category: Shi K]] | |||
[[Category: Tompkins KJ]] | |||
Latest revision as of 15:25, 18 October 2023
Wheat dwarf virus Rep domain circular permutation complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+
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