2brs: Difference between revisions

Revision as of 13:53, 13 December 2023

EMBP Heparin complex

Structural highlights

2brs is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	IDU, SGN, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRG2_HUMAN Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The eosinophil major basic protein (EMBP), a constituent of the eosinophil secondary granule, is implicated in cytotoxicity and mediation of allergic disorders such as asthma. It is a member of the C-type lectin family, but lacks a Ca(2+)- and carbohydrate-binding site as seen in other members of this family. Here, we report the crystal structure of EMBP in complex with a heparin disaccharide and in the absence of Ca(2+), the first such report of any C-lectin with this sugar. We also provide direct evidence of binding of EMBP to heparin and heparin disaccharide by surface plasmon resonance. We propose that the sugars recognized by EMBP are likely to be proteoglycans such as heparin, leading to new interpretations for EMBP function.

Eosinophil-granule major basic protein, a C-type lectin, binds heparin.,Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR Biochemistry. 2005 Nov 1;44(43):14152-8. PMID:16245931^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Overgaard MT, Haaning J, Boldt HB, Olsen IM, Laursen LS, Christiansen M, Gleich GJ, Sottrup-Jensen L, Conover CA, Oxvig C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J Biol Chem. 2000 Oct 6;275(40):31128-33. PMID:10913121 doi:https://dx.doi.org/10.1074/jbc.M001384200
↑ Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR. Eosinophil-granule major basic protein, a C-type lectin, binds heparin. Biochemistry. 2005 Nov 1;44(43):14152-8. PMID:16245931 doi:10.1021/bi051112b

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Overgaard MT, Haaning J, Boldt HB, Olsen IM, Laursen LS, Christiansen M, Gleich GJ, Sottrup-Jensen L, Conover CA, Oxvig C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J Biol Chem. 2000 Oct 6;275(40):31128-33. PMID:10913121 doi:https://dx.doi.org/10.1074/jbc.M001384200

[2] Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR. Eosinophil-granule major basic protein, a C-type lectin, binds heparin. Biochemistry. 2005 Nov 1;44(43):14152-8. PMID:16245931 doi:10.1021/bi051112b

[1]

[2]

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2brs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BRS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=IDU:2-O-SULFO-BETA-L-ALTROPYRANURONIC+ACID'>IDU</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1h8u|1h8u]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IDU:2-O-SULFO-BETA-L-ALTROPYRANURONIC+ACID'>IDU</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2brs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2brs OCA], [https://pdbe.org/2brs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2brs RCSB], [https://www.ebi.ac.uk/pdbsum/2brs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2brs ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PRG2_HUMAN PRG2_HUMAN]] Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.<ref>PMID:10913121</ref>
+[https://www.uniprot.org/uniprot/PRG2_HUMAN PRG2_HUMAN] Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.<ref>PMID:10913121</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 35: @@
 [[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Acharya, K R]]
+[[Category: Acharya KR]]
-[[Category: Gleich, G J]]
+[[Category: Gleich GJ]]
-[[Category: Katsamba, P S]]
+[[Category: Katsamba PS]]
-[[Category: Myszka, D G]]
+[[Category: Myszka DG]]
-[[Category: Ohnuki, L E]]
+[[Category: Ohnuki LE]]
-[[Category: Swaminathan, G J]]
+[[Category: Swaminathan GJ]]
-[[Category: Antibiotic]]
-[[Category: Embp]]
-[[Category: Eosinophil]]
-[[Category: Eosinophil granule protein]]
-[[Category: Glycoprotein]]
-[[Category: Heparin]]
-[[Category: Heparin-binding]]
-[[Category: Immune response]]
-[[Category: Lectin]]
-[[Category: Proteoglycan]]

2brs: Difference between revisions

Revision as of 13:53, 13 December 2023

EMBP Heparin complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

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