Sandbox Reserved 1688: Difference between revisions

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RNA Dependent RNA Polymerase (RdRp) Function –

Nearly all of RNA viruses depend on specific activities performed by RNA dependent RNA polymerases. These activities importantly include the copying of the RNA sequence and facilitating the process of transcription. This is due in part to the fact that NS5B contains no exonuclease or proofreading domain. In NS5B, two divalent cations coordinated by carboxyl groups (as seen in DNA polymerases) catalyze the polymerization of monomers of RNA triphosphates to extend a primer strand, that may have initiated de novo. In the case of NS5B the residues that coordinate divalent cations (Mg2+ or Mn2+ in vitro) are the three ~~<scene name='89/891378/Palmcordmagnesium/3'>~~residues. ~~</scene> Therefore, if~~ a vaccine was created to target the RNA dependent RNA polymerase and inhibit the function of this protein, then the viral genome could not be replicated. If replication halts, the infection cannot spread to other cells.

Nearly all of RNA viruses depend on specific activities performed by RNA dependent RNA polymerases. These activities importantly include the copying of the RNA sequence and facilitating the process of transcription. This is due in part to the fact that NS5B contains no exonuclease or proofreading domain. In NS5B, two divalent cations coordinated by carboxyl groups (as seen in DNA polymerases) catalyze the polymerization of monomers of RNA triphosphates to extend a primer strand, that may have initiated de novo. In the case of NS5B the residues that coordinate divalent cations (Mg2+ or Mn2+ in vitro) are the three aspartate residues. If a vaccine was created to target the RNA dependent RNA polymerase and inhibit the function of this protein, especially affecting active site catalysis, then the viral genome could not be replicated. If replication halts, the infection cannot spread to other cells.

Major Structural Features –

Sandbox Reserved 1688: Difference between revisions

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@@ Line 9: / Line 9: @@
 RNA Dependent RNA Polymerase (RdRp) Function –
-Nearly all of RNA viruses depend on specific activities performed by RNA dependent RNA polymerases. These activities importantly include the copying of the RNA sequence and facilitating the process of transcription. This is due in part to the fact that NS5B contains no exonuclease or proofreading domain. In NS5B, two divalent cations coordinated by carboxyl groups (as seen in DNA polymerases) catalyze the polymerization of monomers of RNA triphosphates to extend a primer strand, that may have initiated de novo. In the case of NS5B the residues that coordinate divalent cations (Mg2+ or Mn2+ in vitro) are the three <scene name='89/891378/Palmcordmagnesium/3'>residues. </scene> Therefore, if a vaccine was created to target the RNA dependent RNA polymerase and inhibit the function of this protein, then the viral genome could not be replicated. If replication halts, the infection cannot spread to other cells.
+Nearly all of RNA viruses depend on specific activities performed by RNA dependent RNA polymerases. These activities importantly include the copying of the RNA sequence and facilitating the process of transcription. This is due in part to the fact that NS5B contains no exonuclease or proofreading domain. In NS5B, two divalent cations coordinated by carboxyl groups (as seen in DNA polymerases) catalyze the polymerization of monomers of RNA triphosphates to extend a primer strand, that may have initiated de novo. In the case of NS5B the residues that coordinate divalent cations (Mg2+ or Mn2+ in vitro) are the three aspartate residues. If a vaccine was created to target the RNA dependent RNA polymerase and inhibit the function of this protein, especially affecting active site catalysis, then the viral genome could not be replicated. If replication halts, the infection cannot spread to other cells.
 Major Structural Features –