Transmembrane protease serine 2: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
No edit summary
No edit summary
Line 7: Line 7:
== Protease activity ==
== Protease activity ==


h TMPRSS2, as a serine protease, cleaves peptide bonds present after positively charged residues (lysine or arginine). The main player in the catalytic mechanism is the catalytic triad formed by His296, Asp345, and Ser441. This three aminoacids are located in the active site of the enzyme. <ref>DOI 10.1073/pnas.87.17.6659</ref>
TMPRSS2, as a serine protease, cleaves peptide bonds present after positively charged residues (lysine or arginine). The main player in the catalytic mechanism is the catalytic triad formed by His296, Asp345, and Ser441. This three aminoacids are located in the active site of the enzyme. <ref>DOI 10.1073/pnas.87.17.6659</ref>


The substrate specificity is achieved with the presence of a negatively charged Asp residue at the bottom of a cavity usually indicated as “S1 specificity pocket”. <ref>DOI 10.1016/j.ejps.2020.105495</ref>
The substrate specificity is achieved with the presence of a negatively charged Asp residue at the bottom of a cavity usually indicated as “S1 specificity pocket”. <ref>DOI 10.1016/j.ejps.2020.105495</ref>
Line 44: Line 44:
=== Protein ===
=== Protein ===


TMPRSS2 is a 492 amino acid single-pass type II membrane protein. This protein is defined by the presence of an N-terminal cytoplasmic domain, a transmembrane helical domain, and three extracellular domains: <ref>DOI 10.1136/jclinpath-2020-206987</ref><scene name='89/897681/Domains/2'>TMPRSS2 protein domains</scene>
TMPRSS2 is a 492 amino acid single-pass type II membrane protein. This protein is defined by the presence of an N-terminal cytoplasmic domain, a transmembrane helical domain (aa 84-106), and three extracellular domains: <ref>DOI 10.1136/jclinpath-2020-206987</ref><scene name='89/897681/Domains/2'>TMPRSS2 protein domains</scene>
*Low-density lipoprotein (LDL)-receptor class A domain: which forms a binding site for calcium rupo
*Low-density lipoprotein (LDL)-receptor class A domain: which forms a binding site for calcium. Expands from amino acid 113 to 148.
*Scavenger receptor cysteine-rich domain (SRCR)  
*Scavenger receptor cysteine-rich domain (SRCR) of group A from amino acid 149 to 242.
*Peptidase S1 domain, also known as serine protease domain (SPD)
*Peptidase S1 domain, also known as serine protease domain (SPD) from amino acid 255 to 492.


[[Image:TMPRSS2.jpg]]
[[Image:TMPRSS2.jpg]]
Retrieved from "https://proteopedia.org/Transmembrane_protease_serine_2"