7fbh: Difference between revisions
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==geranyl pyrophosphate C6-methyltransferase BezA== | ==geranyl pyrophosphate C6-methyltransferase BezA== | ||
<StructureSection load='7fbh' size='340' side='right'caption='[[7fbh]]' scene=''> | <StructureSection load='7fbh' size='340' side='right'caption='[[7fbh]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FBH FirstGlance]. <br> | <table><tr><td colspan='2'>[[7fbh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FBH FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fbh OCA], [https://pdbe.org/7fbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fbh RCSB], [https://www.ebi.ac.uk/pdbsum/7fbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fbh ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fbh OCA], [https://pdbe.org/7fbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fbh RCSB], [https://www.ebi.ac.uk/pdbsum/7fbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fbh ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Prenyl pyrophosphate methyltransferases enhance the structural diversity of terpenoids. However, the molecular basis of their catalytic mechanisms is poorly understood. In this study, using multiple strategies, we characterized a geranyl pyrophosphate (GPP) C6-methyltransferase, BezA. Biochemical analysis revealed that BezA requires Mg(2+) and solely methylates GPP. The crystal structures of BezA and its complex with S-adenosyl homocysteine were solved at 2.10 and 2.56 A, respectively. Further analyses using site-directed mutagenesis, molecular docking, molecular dynamics simulations, and quantum mechanics/molecular mechanics calculations revealed the molecular basis of the methylation reaction. Importantly, the function of E170 as a catalytic base to complete the methylation reaction was established. We also succeeded in switching the substrate specificity by introducing a W210A substitution, resulting in an unprecedented farnesyl pyrophosphate C6-methyltransferase. | |||
Structural and Molecular Basis of the Catalytic Mechanism of Geranyl Pyrophosphate C6-Methyltransferase: Creation of an Unprecedented Farnesyl Pyrophosphate C6-Methyltransferase.,Tsutsumi H, Moriwaki Y, Terada T, Shimizu K, Shin-Ya K, Katsuyama Y, Ohnishi Y Angew Chem Int Ed Engl. 2021 Oct 9. doi: 10.1002/anie.202111217. PMID:34626048<ref>PMID:34626048</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7fbh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Katsuyama Y]] | [[Category: Katsuyama, Y]] | ||
[[Category: Moriwaki Y]] | [[Category: Moriwaki, Y]] | ||
[[Category: Ohnishi Y]] | [[Category: Ohnishi, Y]] | ||
[[Category: Shimizu K]] | [[Category: Shimizu, K]] | ||
[[Category: Terada T]] | [[Category: Terada, T]] | ||
[[Category: Tsutsumi H]] | [[Category: Tsutsumi, H]] | ||
[[Category: Methyltransferase geranyl pyrophosphate s-adenosylmethionine]] | |||
[[Category: Transferase]] | |||
Revision as of 11:00, 16 February 2022
geranyl pyrophosphate C6-methyltransferase BezA
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