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| {{STRUCTURE_1fwi| PDB=1fwi | SCENE= }} | | {{STRUCTURE_1fwi| PDB=1fwi | SCENE= }} |
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| '''KLEBSIELLA AEROGENES UREASE, H134A VARIANT'''
| | ===KLEBSIELLA AEROGENES UREASE, H134A VARIANT=== |
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| ==Overview==
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| A mutant form of Klebsiella aerogenes urease possessing Ala instead of His at position 134 (H134A) is inactive and binds approximately half the normal complement of nickel (Park, I.-S., and Hausinger, R. P.(1993) Protein Sci. 2, 1034-1041). The crystal structure of the H134A protein was obtained at 2.0-A resolution, and it confirms that only Ni-1 of the two nickel ions found in the native enzyme is present. In contrast to the pseudotetrahedral geometry observed for Ni-1 in native urease (where it is liganded by His-246, His-272, one oxygen atom of carbamylated Lys-217, and a water molecule at partial occupancy), the mononickel metallocenter in the H134A protein was found to possess octahedral geometry and was coordinated by the above protein ligands plus three water molecules. The nickel site of H134A urease was probed by UV-visible, variable temperature magnetic circular dichroism, and x-ray absorption spectroscopies. The spectroscopic data are consistent with the presence of Ni(II) in octahedral geometry coordinated by two histidylimidazoles and additional oxygen and/or nitrogen donors. These data underscore the requirement of Ni-2 for formation of active urease and demonstrate the important role of Ni-2 in establishing the proper Ni-1 coordination geometry.
| | The line below this paragraph, {{ABSTRACT_PUBMED_8702515}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8702515 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8702515}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Mutant]] | | [[Category: Mutant]] |
| [[Category: Nickel metalloenzyme]] | | [[Category: Nickel metalloenzyme]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:50:46 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:03:03 2008'' |
