1fx0: Difference between revisions

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[[Image:1fx0.gif|left|200px]]
{{Seed}}
[[Image:1fx0.png|left|200px]]


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{{STRUCTURE_1fx0|  PDB=1fx0  |  SCENE=  }}  
{{STRUCTURE_1fx0|  PDB=1fx0  |  SCENE=  }}  


'''Crystal structure of the chloroplast F1-ATPase from spinach'''
===Crystal structure of the chloroplast F1-ATPase from spinach===




==Overview==
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The structure of the F(1)-ATPase from spinach chloroplasts was determined to 3.2 A resolution by molecular replacement based on the homologous structure of the bovine mitochondrial enzyme. The crystallized complex contains four different subunits in a stoichiometry of alpha(3)beta(3)gammaepsilon. Subunit delta was removed before crystallization to improve the diffraction of the crystals. The overall structure of the noncatalytic alpha-subunits and the catalytic beta-subunits is highly similar to those of the mitochondrial and thermophilic subunits. However, in the crystal structure of the chloroplast enzyme, all alpha- and beta-subunits adopt a closed conformation and appear to contain no bound adenine nucleotides. The superimposed crystallographic symmetry in the space group R32 impaired an exact tracing of the gamma- and epsilon-subunits in the complex. However, clear electron density was present at the core of the alpha(3)beta(3)-subcomplex, which probably represents the C-terminal domain of the gamma-subunit. The structure of the spinach chloroplast F(1) has a potential binding site for the phytotoxin, tentoxin, at the alphabeta-interface near betaAsp(83) and an insertion from betaGly(56)-Asn(60) in the N-terminal beta-barrel domain probably increases the thermal stability of the complex. The structure probably represents an inactive latent state of the ATPase, which is unique to chloroplast and cyanobacterial enzymes.
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{{ABSTRACT_PUBMED_11032839}}


==About this Structure==
==About this Structure==
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[[Category: Potential tentoxin binding site]]
[[Category: Potential tentoxin binding site]]
[[Category: Thermal stability]]
[[Category: Thermal stability]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:51:39 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:04:22 2008''

Revision as of 01:04, 1 July 2008

File:1fx0.png

Template:STRUCTURE 1fx0

Crystal structure of the chloroplast F1-ATPase from spinach

Template:ABSTRACT PUBMED 11032839

About this Structure

1FX0 is a Protein complex structure of sequences from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

The structure of the chloroplast F1-ATPase at 3.2 A resolution., Groth G, Pohl E, J Biol Chem. 2001 Jan 12;276(2):1345-52. PMID:11032839

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