7vg8: Difference between revisions
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==TYLCV Rep-DNA== | |||
<StructureSection load='7vg8' size='340' side='right'caption='[[7vg8]], [[Resolution|resolution]] 2.04Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7vg8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tomato_yellow_leaf_curl_virus Tomato yellow leaf curl virus] and [https://en.wikipedia.org/wiki/Tomato_yellow_leaf_curl_virus-IL_[MX:_SLP:11] Tomato yellow leaf curl virus-IL [MX: SLP:11]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VG8 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vg8 OCA], [https://pdbe.org/7vg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vg8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vg8 ProSAT]</span></td></tr> | ||
[[Category: Wang | </table> | ||
[[Category: Zhang | == Function == | ||
[[https://www.uniprot.org/uniprot/A0A286LJ32_9GEMI A0A286LJ32_9GEMI]] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.[ARBA:ARBA00024923][RuleBase:RU361249] | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tomato yellow leaf curl virus]] | |||
[[Category: Wang CN]] | |||
[[Category: Zhang SY]] | |||
