7ofn: Difference between revisions
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==NMR solution structure of the SYLF domain of Burkholderia pseudomallei BPSL1445== | ==NMR solution structure of the SYLF domain of Burkholderia pseudomallei BPSL1445== | ||
<StructureSection load='7ofn' size='340' side='right'caption='[[7ofn]]' scene=''> | <StructureSection load='7ofn' size='340' side='right'caption='[[7ofn]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full | <table><tr><td colspan='2'>[[7ofn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OFN FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ofn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ofn OCA], [https://pdbe.org/7ofn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ofn RCSB], [https://www.ebi.ac.uk/pdbsum/7ofn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ofn ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ofn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ofn OCA], [https://pdbe.org/7ofn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ofn RCSB], [https://www.ebi.ac.uk/pdbsum/7ofn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ofn ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The SYLF domain is an evolutionary conserved protein domain with phosphatidylinositol binding ability, whose three-dimensional structure is unknown. Here, we present the solution structure and the dynamics characterization of the SYLF domain of the bacterial BPSL1445 protein. BPSL1445 is a seroreactive antigen and a diagnostic marker of Burkholderia pseudomallei, the etiological agent of melioidosis, a severe infectious disease in the tropics. The BPSL1445 SYLF domain (BPSL1445-SYLF) consists of a beta-barrel core, with two flexible loops protruding out of the barrel and three helices packing on its surface. Our structure allows for a more precise definition of the boundaries of the SYLF domain compared to the previously reported one and suggests common ancestry with bacterial EipA domains. We also demonstrate by phosphatidyl-inositol phosphate arrays and nuclear magnetic resonance titrations that BPSL1445-SYLF weakly interacts with phosphoinositides, thus supporting lipid binding abilities of this domain also in prokaryotes. | |||
Solution Structure of the BPSL1445 Protein of Burkholderia pseudomallei Reveals the SYLF Domain Three-Dimensional Fold.,Quilici G, Berardi A, Fabris C, Ghitti M, Punta M, Gourlay LJ, Bolognesi M, Musco G ACS Chem Biol. 2022 Jan 21;17(1):230-239. doi: 10.1021/acschembio.1c00886. Epub, 2021 Dec 30. PMID:34968022<ref>PMID:34968022</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ofn" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Berardi A]] | [[Category: Berardi, A]] | ||
[[Category: Musco G]] | [[Category: Musco, G]] | ||
[[Category: Quilici G]] | [[Category: Quilici, G]] | ||
[[Category: Burkholderia pseudomallei lipoprotein sylf domain beta berrel]] | |||
[[Category: Lipid binding protein]] | |||
Revision as of 07:13, 2 February 2022
NMR solution structure of the SYLF domain of Burkholderia pseudomallei BPSL1445
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