7qia: Difference between revisions

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====

==Structure of apo-EleNRMT in complex with two nanobodies at 3.5A==

== Structural highlights ==

<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>

<table><tr><td colspan='2'>[[7qia]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eggerthella_lenta Eggerthella lenta] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIA FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qia OCA], [https://pdbe.org/7qia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qia RCSB], [https://www.ebi.ac.uk/pdbsum/7qia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qia ProSAT]</span></td></tr>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qia OCA], [https://pdbe.org/7qia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qia RCSB], [https://www.ebi.ac.uk/pdbsum/7qia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qia ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/C8WJ67_EGGLE C8WJ67_EGGLE]

== Publication Abstract from PubMed ==

Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H(+) serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg(2+). The protein transports Mg(2+) and Mn(2+) but not Ca(2+) by a mechanism that is not coupled to H(+). Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell.

Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family.,Ramanadane K, Straub MS, Dutzler R, Manatschal C Elife. 2022 Jan 10;11:e74589. doi: 10.7554/eLife.74589. PMID:35001872<ref>PMID:35001872</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Eggerthella lenta]]

[[Category: Large Structures]]

[[Category: ~~Z-disk~~]]

[[Category: Synthetic construct]]

[[Category: Dutzler R]]

[[Category: Manatschal C]]

[[Category: Ramanadane K]]

[[Category: Straub MS]]

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-====
+==Structure of apo-EleNRMT in complex with two nanobodies at 3.5A==
-<StructureSection load='7qia' size='340' side='right'caption='[[7qia]]' scene=''>
+<StructureSection load='7qia' size='340' side='right'caption='[[7qia]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7qia]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eggerthella_lenta Eggerthella lenta] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIA FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qia OCA], [https://pdbe.org/7qia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qia RCSB], [https://www.ebi.ac.uk/pdbsum/7qia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qia ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qia OCA], [https://pdbe.org/7qia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qia RCSB], [https://www.ebi.ac.uk/pdbsum/7qia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qia ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C8WJ67_EGGLE C8WJ67_EGGLE]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H(+) serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg(2+). The protein transports Mg(2+) and Mn(2+) but not Ca(2+) by a mechanism that is not coupled to H(+). Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell.
+Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family.,Ramanadane K, Straub MS, Dutzler R, Manatschal C Elife. 2022 Jan 10;11:e74589. doi: 10.7554/eLife.74589. PMID:35001872<ref>PMID:35001872</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 7qia" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
+[[Category: Eggerthella lenta]]
 [[Category: Large Structures]]
-[[Category: Z-disk]]
+[[Category: Synthetic construct]]
+[[Category: Dutzler R]]
+[[Category: Manatschal C]]
+[[Category: Ramanadane K]]
+[[Category: Straub MS]]