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==Solution structure of the chimeric Nrd1-Nab3 heterodimerization domains==
==Solution structure of the chimeric Nrd1-Nab3 heterodimerization domains==
<StructureSection load='7prd' size='340' side='right'caption='[[7prd]]' scene=''>
<StructureSection load='7prd' size='340' side='right'caption='[[7prd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRD FirstGlance]. <br>
<table><tr><td colspan='2'>[[7prd]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRD FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7prd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7prd OCA], [https://pdbe.org/7prd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7prd RCSB], [https://www.ebi.ac.uk/pdbsum/7prd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7prd ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7prd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7prd OCA], [https://pdbe.org/7prd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7prd RCSB], [https://www.ebi.ac.uk/pdbsum/7prd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7prd ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Heterodimerization of RNA binding proteins Nrd1 and Nab3 is essential to communicate the RNA recognition in the nascent transcript with the Nrd1 recognition of the Ser5-phosphorylated Rbp1 C-terminal domain in RNA polymerase II. The structure of a Nrd1-Nab3 chimera reveals the basis of heterodimerization, filling a missing gap in knowledge of this system. The free form of the Nrd1 interaction domain of Nab3 (NRID) forms a multi-state three-helix bundle that is clamped in a single conformation upon complex formation with the Nab3 interaction domain of Nrd1 (NAID). The latter domain forms two long helices that wrap around NRID, resulting in an extensive protein-protein interface that would explain the highly favorable free energy of heterodimerization. Mutagenesis of some conserved hydrophobic residues involved in the heterodimerization leads to temperature-sensitive phenotypes, revealing the importance of this interaction in yeast cell fitness. The Nrd1-Nab3 structure resembles the previously reported Rna14/Rna15 heterodimer structure, which is part of the poly(A)-dependent termination pathway, suggesting that both machineries use similar structural solutions despite they share little sequence homology and are potentially evolutionary divergent.
Structural basis of Nrd1-Nab3 heterodimerization.,Chaves-Arquero B, Martinez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jimenez MA, Calvo O, Perez-Canadillas JM Life Sci Alliance. 2022 Jan 12;5(4). pii: 5/4/e202101252. doi:, 10.26508/lsa.202101252. Print 2022 Apr. PMID:35022249<ref>PMID:35022249</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7prd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chaves-Arquero B]]
[[Category: Chaves-Arquero, B]]
[[Category: Martinez-Lumbreras S]]
[[Category: Martinez-Lumbreras, S]]
[[Category: Perez-Canadillas JM]]
[[Category: Perez-Canadillas, J M]]
[[Category: Chimera]]
[[Category: Heterodimerization]]
[[Category: Nab3]]
[[Category: Nns pathway]]
[[Category: Nrd1]]
[[Category: Transcription]]
[[Category: Transcription termination]]
[[Category: Yeast]]
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