Sandbox Reserved 1091: Difference between revisions
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== History == | == History == | ||
''Aeromonas sobria'' has been isolated for the first time from sick Rana Catesbeiana, also named bullfrog. Bullfrog is a species of frog coming from North America. The discovery of a particular ''A.sobria'' | ''Aeromonas sobria'' has been isolated for the first time from sick Rana Catesbeiana, also named bullfrog. Bullfrog is a species of frog coming from North America. The discovery of a particular ''A.sobria'' 08005 was made in Xiamen in China in 1981. It was the first time the sequencing of ''A.sobria'' 08005 had been made. | ||
<ref>Draft Genome Sequence of Aeromonas sobria Strain 08005, Isolated from Sick Rana Catesbeiana, Qiu-Hua Yang, ASM Journals, Genome Announcements, | <ref>Draft Genome Sequence of Aeromonas sobria Strain 08005, Isolated from Sick Rana Catesbeiana, Qiu-Hua Yang, ASM Journals, Genome Announcements, | ||
Vol. 5, No. 2, (2017)</ref> | Vol. 5, No. 2, (2017)</ref> | ||
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The '''peptide bonds''' were shown to be cleaved when two basic residues were in sequence. A Lys residue at positions P1 and P2 relative to the cleavage site is prefered. If an Arg residue is at P4 position the substrate cleavage will be enhanced. <ref>Cleavage specificity of serine protease of Aeromonas sobria, a member of the kexin family of subtilases., H. Kobayashi, Okayama University, Japan,FEMS Microbiology Letters, Volume 256, Issue 1, March 2006, Pages 165–170,</ref> | The '''peptide bonds''' were shown to be cleaved when two basic residues were in sequence. A Lys residue at positions P1 and P2 relative to the cleavage site is prefered. If an Arg residue is at P4 position the substrate cleavage will be enhanced. <ref>Cleavage specificity of serine protease of Aeromonas sobria, a member of the kexin family of subtilases., H. Kobayashi, Okayama University, Japan,FEMS Microbiology Letters, Volume 256, Issue 1, March 2006, Pages 165–170,</ref> | ||
The protease activity is strongly attenuated by serine protease inhibitors ([http://en.wikipedia.org/wiki/Diisopropyl_fluorophosphate DFP], [http://fr.wikipedia.org/wiki/Fluorure_de_4-(2-aminoéthyl)benzènesulfonyle AEBSF]). Moreover, a soybean [http://en.wikipedia.org/wiki/Trypsin_inhibitor trypsin inhibitor] was shown not to block the proteolytic action of ASP itself but could inhibit the [http://en.m.wikipedia.org/wiki/Vascular_permeability vascular permeability] enhancing activity that follows after injection of ASP into epithelial cells. <ref>Physicochemical and biological properties od an extracellular serine protease od Aeromonas sobria. Ritsuko Yokoyama, Yoshio Fujii et al. Japan (2002)</ref> | |||
The experimental finding suggests that epithelial trypsin-like proteases mediate the reaction causing enhanced vascular permeability. It is likely that ASP stimulates the secretion and maturation of epithelial trypsin proteases, thus enhancing vascular permeability. ASP could stimulate the [http://en.wikipedia.org/wiki/Bradykinin bradykinin]-releasing pathway, thus stimulating mast cells to release histamine and further enhance the vascular permeability. | The experimental finding suggests that epithelial trypsin-like proteases mediate the reaction causing enhanced vascular permeability. It is likely that ASP stimulates the secretion and maturation of epithelial trypsin proteases, thus enhancing vascular permeability. ASP could stimulate the [http://en.wikipedia.org/wiki/Bradykinin bradykinin]-releasing pathway, thus stimulating mast cells to release histamine and further enhance the vascular permeability. | ||
