Sandbox Reserved 1657: Difference between revisions
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<scene name='86/868190/5czx_version_2_with_ligands/1'>5CZX</scene> stands for the <scene name='86/868190/Nrr_domain_alonecentre/4'>NRR (negative regulatory region)</scene> of the [https://www.uniprot.org/uniprot/Q9UM47 neurogenic locus notch homolog protein 3] in complex with 20358 [https://en.wikipedia.org/wiki/Fragment_antigen-binding Fab]. This NRR is made of <scene name='86/868190/4zlp_2_chains_nrr/1'>2 identical chain structures</scene> encoded by the [https://en.wikipedia.org/wiki/Notch_3 NOTCH3 gene](In this article, we will only focus on one of the two chains). It is composed of <scene name='86/868190/Lnr_domains/1'>three LIN12/Notch repeats and linker</scene> (LNRs respectively <scene name='86/868190/Lna_domain/1'>LNR-A</scene>,<scene name='86/868190/Lnb_domain/1'>LNR-B</scene>, <scene name='86/868190/Lrn_linker_bc/1'>LNR-B/C linker</scene> and <scene name='86/868190/Lnc/1'>LNR-C</scene> ) and a <scene name='86/868190/Hd/1'>heterodimerization domain (HD)</scene> composed by <scene name='86/868190/Hd-c/1'>HD-C</scene> and <scene name='86/868190/Hd-n/1'>HD-N</scene>.<ref name="oncogene">Bernasconi-Elias, P., Hu, T., Jenkins, D. et al. Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies. Oncogene 35, 6077–6086 (2016). https://doi.org/10.1038/onc.2016.133</ref>. Full experimental information is available on [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5czx OCA]. For a guided tour on the structure components in complex with [https://swissmodel.expasy.org/templates/5czx.2 20358 Fab] use [https://proteopedia.org/wiki/fgij/fg.htm?mol=5CZX FirstGlance]. | <scene name='86/868190/5czx_version_2_with_ligands/1'>5CZX</scene> stands for the <scene name='86/868190/Nrr_domain_alonecentre/4'>NRR (negative regulatory region)</scene> of the [https://www.uniprot.org/uniprot/Q9UM47 neurogenic locus notch homolog protein 3] in complex with 20358 [https://en.wikipedia.org/wiki/Fragment_antigen-binding Fab]. This NRR is made of <scene name='86/868190/4zlp_2_chains_nrr/1'>2 identical chain structures</scene> encoded by the [https://en.wikipedia.org/wiki/Notch_3 NOTCH3 gene](In this article, we will only focus on one of the two chains). It is composed of <scene name='86/868190/Lnr_domains/1'>three LIN12/Notch repeats and linker</scene> (LNRs respectively <scene name='86/868190/Lna_domain/1'>LNR-A</scene>,<scene name='86/868190/Lnb_domain/1'>LNR-B</scene>, <scene name='86/868190/Lrn_linker_bc/1'>LNR-B/C linker</scene> and <scene name='86/868190/Lnc/1'>LNR-C</scene> ) and a <scene name='86/868190/Hd/1'>heterodimerization domain (HD)</scene> composed by <scene name='86/868190/Hd-c/1'>HD-C</scene> and <scene name='86/868190/Hd-n/1'>HD-N</scene>.<ref name="oncogene">Bernasconi-Elias, P., Hu, T., Jenkins, D. et al. Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies. Oncogene 35, 6077–6086 (2016). https://doi.org/10.1038/onc.2016.133</ref>. Full experimental information is available on [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5czx OCA]. For a guided tour on the structure components in complex with [https://swissmodel.expasy.org/templates/5czx.2 20358 Fab] use [https://proteopedia.org/wiki/fgij/fg.htm?mol=5CZX FirstGlance]. | ||
The X-Ray crystallized structure 5CZX was realized and published in the same article (Article reference<ref name="oncogene">Bernasconi-Elias, P., Hu, T., Jenkins, D. et al. Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies. Oncogene 35, 6077–6086 (2016). https://doi.org/10.1038/onc.2016.133</ref>) as [https://www.rcsb.org/structure/5CZV 5CZV] (Notch 3 NRR in complex with 20350) in order to exhibit the areas | The X-Ray crystallized structure 5CZX was realized and published in the same article (Article reference<ref name="oncogene">Bernasconi-Elias, P., Hu, T., Jenkins, D. et al. Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies. Oncogene 35, 6077–6086 (2016). https://doi.org/10.1038/onc.2016.133</ref>) as [https://www.rcsb.org/structure/5CZV 5CZV] (Notch 3 NRR in complex with 20350) in order to exhibit the areas where the studied antibody (MOR 20358 in the case of 5CZX) bound to the NRR in order to draw conclusions regarding their Notch3 signalling inhibitory properties. | ||
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