1ddj: Difference between revisions

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[[Image:1ddj.gif|left|200px]]<br />

[[Image:1ddj.gif|left|200px]]<br /><applet load="1ddj" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1ddj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1ddj, resolution 2.0Å" />

'''CRYSTAL STRUCTURE OF HUMAN PLASMINOGEN CATALYTIC DOMAIN'''<br />

==Overview==

Activation of the serine protease plasmin from its zymogen, plasminogen, is the key step in fibrinolysis leading to blood clot dissolution. It also, plays critical roles in cell migration, such as in tumor metastasis. Here, we report the crystal structure of an inactive S741A mutant of human, plasminogen catalytic domain at 2.0 A resolution. This structure permits a, direct comparison with that of the plasmin catalytic unit. Unique, conformational differences are present between these two structures that, are not seen in other zymogen-enzyme pairs of the trypsin family. The, functional significance of these differences and the structural basis of, plasminogen activation is discussed in the light of this new structure.

Activation of the serine protease plasmin from its zymogen, plasminogen, is the key step in fibrinolysis leading to blood clot dissolution. It also plays critical roles in cell migration, such as in tumor metastasis. Here, we report the crystal structure of an inactive S741A mutant of human plasminogen catalytic domain at 2.0 A resolution. This structure permits a direct comparison with that of the plasmin catalytic unit. Unique conformational differences are present between these two structures that are not seen in other zymogen-enzyme pairs of the trypsin family. The functional significance of these differences and the structural basis of plasminogen activation is discussed in the light of this new structure.

==Disease==

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==About this Structure==

1DDJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DDJ OCA].

1DDJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDJ OCA].

==Reference==

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[[Category: plasminogen]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov~~ 12 16:31:~~13 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:32 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ddj.gif|left|200px]]<br />
+[[Image:1ddj.gif|left|200px]]<br /><applet load="1ddj" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1ddj" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1ddj, resolution 2.0&Aring;" />
 '''CRYSTAL STRUCTURE OF HUMAN PLASMINOGEN CATALYTIC DOMAIN'''<br />
 ==Overview==
-Activation of the serine protease plasmin from its zymogen, plasminogen, is the key step in fibrinolysis leading to blood clot dissolution. It also, plays critical roles in cell migration, such as in tumor metastasis. Here, we report the crystal structure of an inactive S741A mutant of human, plasminogen catalytic domain at 2.0 A resolution. This structure permits a, direct comparison with that of the plasmin catalytic unit. Unique, conformational differences are present between these two structures that, are not seen in other zymogen-enzyme pairs of the trypsin family. The, functional significance of these differences and the structural basis of, plasminogen activation is discussed in the light of this new structure.
+Activation of the serine protease plasmin from its zymogen, plasminogen, is the key step in fibrinolysis leading to blood clot dissolution. It also plays critical roles in cell migration, such as in tumor metastasis. Here, we report the crystal structure of an inactive S741A mutant of human plasminogen catalytic domain at 2.0 A resolution. This structure permits a direct comparison with that of the plasmin catalytic unit. Unique conformational differences are present between these two structures that are not seen in other zymogen-enzyme pairs of the trypsin family. The functional significance of these differences and the structural basis of plasminogen activation is discussed in the light of this new structure.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-DDJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DDJ OCA].
+DDJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDJ OCA].
 ==Reference==
@@ Line 27: / Line 26: @@
 [[Category: plasminogen]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:31:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:32 2008''