1dg3: Difference between revisions

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[[Image:1dg3.gif|left|200px]]<br />

[[Image:1dg3.gif|left|200px]]<br /><applet load="1dg3" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1dg3" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1dg3, resolution 1.80Å" />

'''STRUCTURE OF HUMAN GUANYLATE BINDING PROTEIN-1 IN NUCLEOTIDE FREE FORM'''<br />

==Overview==

Interferon-gamma is an immunomodulatory substance that induces the, expression of many genes to orchestrate a cellular response and establish, the antiviral state of the cell. Among the most abundant antiviral, proteins induced by interferon-gamma are guanylate-binding proteins such, as GBP1 and GBP2. These are large GTP-binding proteins of relative, molecular mass 67,000 with a high-turnover GTPase activity and an, antiviral effect. Here we have determined the crystal structure of, full-length human GBP1 to 1.8 A resolution. The amino-terminal 278, residues constitute a modified G domain with a number of insertions, compared to the canonical Ras structure, and the carboxy-terminal part is, an extended helical domain with unique features. From the structure and, biochemical experiments reported here, GBP1 appears to belong to the group, of large GTP-binding proteins that includes Mx and dynamin, the common, property of which is the ability to undergo oligomerization with a high, concentration-dependent GTPase activity.

Interferon-gamma is an immunomodulatory substance that induces the expression of many genes to orchestrate a cellular response and establish the antiviral state of the cell. Among the most abundant antiviral proteins induced by interferon-gamma are guanylate-binding proteins such as GBP1 and GBP2. These are large GTP-binding proteins of relative molecular mass 67,000 with a high-turnover GTPase activity and an antiviral effect. Here we have determined the crystal structure of full-length human GBP1 to 1.8 A resolution. The amino-terminal 278 residues constitute a modified G domain with a number of insertions compared to the canonical Ras structure, and the carboxy-terminal part is an extended helical domain with unique features. From the structure and biochemical experiments reported here, GBP1 appears to belong to the group of large GTP-binding proteins that includes Mx and dynamin, the common property of which is the ability to undergo oligomerization with a high concentration-dependent GTPase activity.

==About this Structure==

1DG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DG3 OCA].

1DG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG3 OCA].

==Reference==

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[[Category: Single protein]]

[[Category: Herrmann, C.]]

[[Category: Praefcke, G.J.K.]]

[[Category: Praefcke, G J.K.]]

[[Category: Prakash, B.]]

[[Category: Renault, L.]]

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[[Category: signaling protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov~~ 12 16:~~32:02 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:18 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1dg3.gif|left|200px]]<br />
+[[Image:1dg3.gif|left|200px]]<br /><applet load="1dg3" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1dg3" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1dg3, resolution 1.80&Aring;" />
 '''STRUCTURE OF HUMAN GUANYLATE BINDING PROTEIN-1 IN NUCLEOTIDE FREE FORM'''<br />
 ==Overview==
-Interferon-gamma is an immunomodulatory substance that induces the, expression of many genes to orchestrate a cellular response and establish, the antiviral state of the cell. Among the most abundant antiviral, proteins induced by interferon-gamma are guanylate-binding proteins such, as GBP1 and GBP2. These are large GTP-binding proteins of relative, molecular mass 67,000 with a high-turnover GTPase activity and an, antiviral effect. Here we have determined the crystal structure of, full-length human GBP1 to 1.8 A resolution. The amino-terminal 278, residues constitute a modified G domain with a number of insertions, compared to the canonical Ras structure, and the carboxy-terminal part is, an extended helical domain with unique features. From the structure and, biochemical experiments reported here, GBP1 appears to belong to the group, of large GTP-binding proteins that includes Mx and dynamin, the common, property of which is the ability to undergo oligomerization with a high, concentration-dependent GTPase activity.
+Interferon-gamma is an immunomodulatory substance that induces the expression of many genes to orchestrate a cellular response and establish the antiviral state of the cell. Among the most abundant antiviral proteins induced by interferon-gamma are guanylate-binding proteins such as GBP1 and GBP2. These are large GTP-binding proteins of relative molecular mass 67,000 with a high-turnover GTPase activity and an antiviral effect. Here we have determined the crystal structure of full-length human GBP1 to 1.8 A resolution. The amino-terminal 278 residues constitute a modified G domain with a number of insertions compared to the canonical Ras structure, and the carboxy-terminal part is an extended helical domain with unique features. From the structure and biochemical experiments reported here, GBP1 appears to belong to the group of large GTP-binding proteins that includes Mx and dynamin, the common property of which is the ability to undergo oligomerization with a high concentration-dependent GTPase activity.
 ==About this Structure==
-DG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DG3 OCA].
+DG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG3 OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Herrmann, C.]]
-[[Category: Praefcke, G.J.K.]]
+[[Category: Praefcke, G J.K.]]
 [[Category: Prakash, B.]]
 [[Category: Renault, L.]]
@@ Line 28: / Line 27: @@
 [[Category: signaling protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:32:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:18 2008''