1g8t: Difference between revisions

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[[Image:1g8t.jpg|left|200px]]
{{Seed}}
[[Image:1g8t.png|left|200px]]


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{{STRUCTURE_1g8t|  PDB=1g8t  |  SCENE=  }}  
{{STRUCTURE_1g8t|  PDB=1g8t  |  SCENE=  }}  


'''SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION'''
===SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION===




==Overview==
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The three-dimensional crystal structure of Serratia marcescens endonuclease has been refined at 1.1 A resolution to an R factor of 12.9% and an R(free) of 15.6% with the use of anisotropic temperature factors. The model contains 3694 non-H atoms, 715 water molecules, four sulfate ions and two Mg(2+)-binding sites at the active sites of the homodimeric protein. It is shown that the magnesium ion linked to the active-site Asn119 of each monomer is surrounded by five water molecules and shows an octahedral coordination geometry. The temperature factors for the bound Mg(2+) ions in the A and B subunits are 7.08 and 4.60 A(2), respectively, and the average temperature factors for the surrounding water molecules are 12.13 and 10.3 A(2), respectively. In comparison with earlier structures, alternative side-chain conformations are defined for 51 residues of the dimer, including the essential active-site residue Arg57. A plausible mechanism of enzyme function is proposed based on the high-resolution S. marcescens nuclease structure, the functional characteristics of the natural and mutational forms of the enzyme and consideration of its structural analogy with homing endo-nuclease I-PpoI.
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{{ABSTRACT_PUBMED_10771425}}


==About this Structure==
==About this Structure==
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[[Category: Nuclease]]
[[Category: Nuclease]]
[[Category: Signal]]
[[Category: Signal]]
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Revision as of 01:53, 1 July 2008

File:1g8t.png

Template:STRUCTURE 1g8t

SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT 1.1 A RESOLUTION

Template:ABSTRACT PUBMED 10771425

About this Structure

1G8T is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.

Reference

Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanism., Shlyapnikov SV, Lunin VV, Perbandt M, Polyakov KM, Lunin VY, Levdikov VM, Betzel C, Mikhailov AM, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):567-72. PMID:10771425

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