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| {{STRUCTURE_1gcj| PDB=1gcj | SCENE= }} | | {{STRUCTURE_1gcj| PDB=1gcj | SCENE= }} |
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| '''N-TERMINAL FRAGMENT OF IMPORTIN-BETA'''
| | ===N-TERMINAL FRAGMENT OF IMPORTIN-BETA=== |
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| ==Overview==
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| Importin-beta is a nuclear transport factor which mediates the nuclear import of various nuclear proteins. The N-terminal 1-449 residue fragment of mouse importin-beta (impbeta449) possesses the ability to bidirectionally translocate through the nuclear pore complex (NPC), and to bind RanGTP. The structure of the uncomplexed form of impbeta449 has been solved at a 2.6 A resolution by X-ray crystallography. It consists of ten copies of the tandemly arrayed HEAT repeat and exhibits conformational flexibility which is involved in protein-protein interaction for nuclear transport. The overall conformation of the HEAT repeats shows that a twisted motion produces a significantly varied superhelical architecture from the previously reported structure of RanGTP-bound importin-beta. These conformational changes appear to be the sum of small conformational changes throughout the polypeptide. Such a flexibility, which resides in the stacked HEAT repeats, is essential for interaction with RanGTP or with NPCs. Furthermore, it was found that impbeta449 has a structural similarity with another nuclear migrating protein, namely beta-catenin, which is composed of another type of helix-repeated structure of ARM repeat. Interestingly, the essential regions for NPC translocation for both importin-beta and beta-catenin are spatially well overlapped with one another. This strongly indicates the importance of helix stacking of the HEAT or ARM repeats for NPC-passage.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10964573}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10964573 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10964573}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Nuclear import factor]] | | [[Category: Nuclear import factor]] |
| [[Category: Nuclear pore-targeting complex component]] | | [[Category: Nuclear pore-targeting complex component]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:24:54 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:04:56 2008'' |
